ProtParam
User-provided sequence:
10 20 30 40 50 60
MGDQALSFLK DFLAGGIAAA VSKTAVAPIE RVKLLLQVQH ASKQISAEKQ YKGIIDCVVR
70 80 90 100 110 120
IPKEQGFLSF WRGNLANVIR YFPTQALNFA FKDKYKQIFL GGVDRHKQFW RYFAGNLASG
130 140 150 160 170 180
GAAGATSLCF VYPLDFARTR LAADVGKGSS QREFNGLGDC LTKIFKSDGL KGLYQGFSVS
190 200 210 220 230 240
VQGIIIYRAA YFGVYDTAKG MLPDPKNVHI IVSWMIAQSV TAVAGLVSYP FDTVRRRMMM
250 260 270 280 290
QSGRKGADIM YTGTLDCWRK IAKDEGANAF FKGAWSNVLR GMGGAFVLVL YDEIKKYV
References and
documentation are available.
Number of amino acids: 298
Molecular weight: 32904.27
Theoretical pI: 9.73
Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 1503
Hydrogen H 2338
Nitrogen N 402
Oxygen O 405
Sulfur S 12
Formula: C
1503H
2338N
402O
405S
12
Total number of atoms: 4660
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 47120
Abs 0.1% (=1 g/l) 1.432, assuming all pairs of Cys residues form cystines
Ext. coefficient 46870
Abs 0.1% (=1 g/l) 1.424, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 20.25
This classifies the protein as stable.
Aliphatic index: 88.09
Grand average of hydropathicity (GRAVY): 0.056