ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGENHDHEQS IKRNSMIYNE NERQLCNSNL KILQNKRALS KNDSSSKQQV QDSKPRRALT 

        70         80         90        100        110        120 
DVPVNNNPLS QNKRIVAGSK AAKVRREENI RPIVSAVQKR QIYNDRTAAE QEEEEEEEGE 

       130        140        150        160        170        180 
DDDAASIVNK KRRIDAEGVS EIVGWQDLDY VEKDDTAMVA EYSAEIFAFL YRRELETLPS 

       190        200        210        220        230        240 
HNYLLDKTSK YYLRPSMRTI LVDWLVEVHE KFQCYPETLF LSINLMDRFL AKNKVTMNKL 

       250        260        270        280        290        300 
QLLAVTSLFI AAKFEEVNLP KLAEYAYITD GAASKNDIKN AEMFMLTSLE FNIGWPNPLN 

       310        320        330        340        350        360 
FLRRISKADD YDPVNRNIGK FILEYAYCCH QFIHLPPSTV SAMAMYIARR MTNRNKNELW 

       370        380        390        400        410        420 
NGTLQHYSGG IDPIHDEAFQ SLCIDLVKDI ASSKTHLDSL ILKYKKPRYG SVYFQTFKWC 

       430 
TSEMHSNFQN LFNLK 

References and documentation are available.

Number of amino acids: 435

Molecular weight: 50431.23

Theoretical pI: 6.58

Amino acid composition: 
Ala (A)  31	  7.1%
Arg (R)  25	  5.7%
Asn (N)  34	  7.8%
Asp (D)  26	  6.0%
Cys (C)   6	  1.4%
Gln (Q)  18	  4.1%
Glu (E)  34	  7.8%
Gly (G)  12	  2.8%
His (H)  10	  2.3%
Ile (I)  27	  6.2%
Leu (L)  40	  9.2%
Lys (K)  33	  7.6%
Met (M)  12	  2.8%
Phe (F)  17	  3.9%
Pro (P)  15	  3.4%
Ser (S)  32	  7.4%
Thr (T)  17	  3.9%
Trp (W)   5	  1.1%
Tyr (Y)  19	  4.4%
Val (V)  22	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 58

Atomic composition:

Carbon      C	      2233
Hydrogen    H	      3506
Nitrogen    N	       620
Oxygen      O	       676
Sulfur      S	        18

Formula: C2233H3506N620O676S18
Total number of atoms: 7053

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    56185
Abs 0.1% (=1 g/l)   1.114, assuming all pairs of Cys residues form cystines


Ext. coefficient    55810
Abs 0.1% (=1 g/l)   1.107, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 56.83
This classifies the protein as unstable.



Aliphatic index: 81.86

Grand average of hydropathicity (GRAVY): -0.583