ProtParam
User-provided sequence:
10 20 30 40 50 60
MGESLDRCID DINRAVDSMS TLYFKPPGIF HNAILQGASN KASIRKDITR LIKDCNHDEA
70 80 90 100 110 120
YLLFKVNPEK QSVSRRDGKE GVFDYVIKRD TDMKRNRRLG RPGEKPIIHV PKEVYLNKDR
130 140 150 160 170 180
LDLNNKRRRT ATTSGGGLNG FIFDTDLIGS SVISNSSSGT FKALSAVFKD DPQIQRLLYA
190 200 210 220 230 240
LENGSVLMEE ESNNQRRKTI FVEDFPTDLI LKVMAEVTDL WPLTEFKQDY DQLYHNYEQL
250 260 270 280 290
SSKLRFIKKE VLLQDDRLKT MSQYHPSSSH DVAKIIRKEK DEIRRLEMEI ANLQE
References and
documentation are available.
Number of amino acids: 295
Molecular weight: 34077.74
Theoretical pI: 8.33
Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 48
Atomic composition:
Carbon C 1498
Hydrogen H 2409
Nitrogen N 429
Oxygen O 460
Sulfur S 9
Formula: C
1498H
2409N
429O
460S
9
Total number of atoms: 4805
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 19035
Abs 0.1% (=1 g/l) 0.559, assuming all pairs of Cys residues form cystines
Ext. coefficient 18910
Abs 0.1% (=1 g/l) 0.555, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 53.62
This classifies the protein as unstable.
Aliphatic index: 84.92
Grand average of hydropathicity (GRAVY): -0.665