ProtParam
User-provided sequence:
10 20 30 40 50 60
MGFNIAMLCD FFYPQLGGVE FHIYHLSQKL IDLGHSVVII THAYKDRVGV RHLTNGLKVY
70 80 90 100 110 120
HVPFFVIFRE TTFPTVFSTF PIIRNILLRE QIQIVHSHGS ASTFAHEGIL HANTMGLRTV
130 140 150 160 170 180
FTDHSLYGFN NLTSIWVNKL LTFTLTNIDR VICVSNTCKE NMIVRTELSP DIISVIPNAV
190 200 210 220 230 240
VSEDFKPRDP TGGTKRKQSR DKIVIVVIGR LFPNKGSDLL TRIIPKVCSS HEDVEFIVAG
250 260 270 280 290 300
DGPKFIDFQQ MIESHRLQKR VQLLGSVPHE KVRDVLCQGD IYLHASLTEA FGTILVEAAS
310 320 330 340 350 360
CNLLIVTTQV GGIPEVLPNE MTVYAEQTSV SDLVQATNKA INIIRSKALD TSSFHDSVSK
370 380 390 400 410 420
MYDWMDVAKR TVEIYTNISS TSSADDKDWM KMVANLYKRD GIWAKHLYLL CGIVEYMLFF
430 440 450
LLEWLYPRDE IDLAPKWPKK TVSNETKEAR ET
References and
documentation are available.
Number of amino acids: 452
Molecular weight: 51242.22
Theoretical pI: 6.79
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 47
Atomic composition:
Carbon C 2317
Hydrogen H 3645
Nitrogen N 613
Oxygen O 661
Sulfur S 18
Formula: C
2317H
3645N
613O
661S
18
Total number of atoms: 7254
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 52745
Abs 0.1% (=1 g/l) 1.029, assuming all pairs of Cys residues form cystines
Ext. coefficient 52370
Abs 0.1% (=1 g/l) 1.022, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.93
This classifies the protein as stable.
Aliphatic index: 99.98
Grand average of hydropathicity (GRAVY): 0.006