ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGFRKILASK SHHSRHHNQH HKNLKLQNHR YVLISNITGS HETKYLSPFR MDNCSGSRRR 

        70         80         90        100        110        120 
DRLHVKLKSL RNKIHKQLHP NCRFDDATKT SDDKCVSYEV PERDGLATIS LEEVFPKSNR 

       130        140        150        160        170        180 
CQIPEENLGE TDSVIHRDLG NFANENDYPQ WRKVESQYNL ENVQPEEDEI VDRLRSEIRS 

       190        200        210        220        230        240 
TKLKSVKTTS RTLEKAIEAR CTGKRVLQQL SCQSNQLTKI ESNCDMLKIQ SNVADRKIDE 

       250        260        270        280        290        300 
LAHENRSLLA LKSPNPFRKK REREKRDQIY NLKLKHRHLQ QETMKRAQDS DKNLAINLSS 

       310        320        330        340        350        360 
EYGRYGQGVE RQRILRDAQK YQFEADEEDN QMEIDLYGNL EQIKAVSGDL KIMAHAFGRE 

       370        380        390 
FEAQNTRMFD IENNVQQADN ALQAKRYRLE KVIGKRW 

References and documentation are available.

Number of amino acids: 397

Molecular weight: 46621.50

Theoretical pI: 9.28

Amino acid composition: 
Ala (A)  20	  5.0%
Arg (R)  37	  9.3%
Asn (N)  28	  7.1%
Asp (D)  25	  6.3%
Cys (C)   7	  1.8%
Gln (Q)  26	  6.5%
Glu (E)  34	  8.6%
Gly (G)  14	  3.5%
His (H)  16	  4.0%
Ile (I)  21	  5.3%
Leu (L)  36	  9.1%
Lys (K)  35	  8.8%
Met (M)   7	  1.8%
Phe (F)  10	  2.5%
Pro (P)   9	  2.3%
Ser (S)  29	  7.3%
Thr (T)  14	  3.5%
Trp (W)   2	  0.5%
Tyr (Y)  11	  2.8%
Val (V)  16	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 72

Atomic composition:

Carbon      C	      2005
Hydrogen    H	      3243
Nitrogen    N	       631
Oxygen      O	       624
Sulfur      S	        14

Formula: C2005H3243N631O624S14
Total number of atoms: 6517

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27765
Abs 0.1% (=1 g/l)   0.596, assuming all pairs of Cys residues form cystines


Ext. coefficient    27390
Abs 0.1% (=1 g/l)   0.587, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.90
This classifies the protein as unstable.



Aliphatic index: 72.72

Grand average of hydropathicity (GRAVY): -1.072