ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGFWENNKDS ITSGLKSAGK YGYQGTKYVA KTGYKASKKH YNNSKARRER KSGKKNSSDE 

        70         80         90        100        110        120 
EYDSEDEMEY ERKPTDIRSL KDPKSFPPPP LKPGQKTYTG QQQQQMPNGQ ASYAFQGAYQ 

       130        140        150        160        170        180 
GQPGAGSTEQ SQYAQPQYNQ YPQQQLQQGV MPQQQQLQQG VVPQQPPIYG EQVPPYGSNS 

       190        200        210        220        230        240 
NATSYQSLPQ QNQPQNAIPS QVSLNSASQQ STGFVSQNLQ YGTQSSNPAP SPSFQNGLQC 

       250        260        270        280        290        300 
HQQPQYVSHG STNLGQSQFP SGQQQQPTTQ FGQQVLPSPA QPQQQQQGQP LPPPRGQVIL 

       310        320        330        340        350        360 
PAPGEPLSNG FGQQQQQQQQ QQQPLNQNNA LLPQMNVEGV SGMAAVQPVY GQAMSSTTNM 

       370        380        390        400        410        420 
QDSNPSYGAS PMQGQPPVGG QPPVPVRMQP QPPQPMQQGN IYPIEPSLDS TGSTPHFEVT 

       430        440        450        460        470        480 
PFDPDAPAPK PKIDIPTVDV SSLPPPPTHR DRGAVVHQEP APSGKIQPNT TSSAASLPAK 

       490        500        510        520        530        540 
HSRTTTADNE RNSGNKENDE STSKSSILGH YDVDANIMPP PKPFRHGLDS VPSEHTTKNA 

       550        560        570        580        590        600 
PERAVPILPP RNNVEPPPPP SRGNFERTES VLSTNAANVQ EDPISNFLPP PKPFRHTETK 

       610        620        630        640        650        660 
QNQNSKASPV EMKGEVLPGH PSEEDRNVEP SLVPQSKPQS QSQFRRAHME TQPIQNFQPP 

       670        680        690        700        710        720 
PKPFRRSQSS NSSDSSYTID GPEANHGRGR GRIAKHHDGD EYNPKSENST ENGRLGDAPN 

       730        740        750        760        770        780 
SFIRKRAPTP PAPSRSEKLH EGTITSEVDS SKDANKYEKS IPPVTSSIQA QQSTKKAPPP 

       790        800        810        820        830        840 
VVKPKPRNFS LKANEYPKEL TREATGQDEV LNSITNELSH IKLRKTNVNL EKLGGSKKVK 

       850        860        870        880        890        900 
DSSPVPSDLD EKYVSASGSI TPPRPPPSRS SPKKVPPVVP KKNDNLKKKP PVVPKKKPLL 

       910        920        930        940 
KSLEPRPIEM ERAYSGDISA ADDNLNPFER YKRNVVPQED DRLHKLK 

References and documentation are available.

Number of amino acids: 947

Molecular weight: 103834.79

Theoretical pI: 9.25

Amino acid composition: 
Ala (A)  49	  5.2%
Arg (R)  39	  4.1%
Asn (N)  59	  6.2%
Asp (D)  37	  3.9%
Cys (C)   1	  0.1%
Gln (Q) 105	 11.1%
Glu (E)  51	  5.4%
Gly (G)  60	  6.3%
His (H)  19	  2.0%
Ile (I)  26	  2.7%
Leu (L)  44	  4.6%
Lys (K)  65	  6.9%
Met (M)  15	  1.6%
Phe (F)  20	  2.1%
Pro (P) 131	 13.8%
Ser (S) 103	 10.9%
Thr (T)  45	  4.8%
Trp (W)   1	  0.1%
Tyr (Y)  29	  3.1%
Val (V)  48	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 88
Total number of positively charged residues (Arg + Lys): 104

Atomic composition:

Carbon      C	      4503
Hydrogen    H	      7084
Nitrogen    N	      1332
Oxygen      O	      1465
Sulfur      S	        16

Formula: C4503H7084N1332O1465S16
Total number of atoms: 14400

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48710
Abs 0.1% (=1 g/l)   0.469, assuming all pairs of Cys residues form cystines


Ext. coefficient    48710
Abs 0.1% (=1 g/l)   0.469, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 69.43
This classifies the protein as unstable.



Aliphatic index: 48.70

Grand average of hydropathicity (GRAVY): -1.158