ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGGCVSSNLL TPTDDSSFSH LTSSSLSQHF VKLTSTTYGL LNLDSSSPSP PSPSSSSSAK 

        70         80         90        100        110        120 
SVITPMTPPE RFTINGKEAA MMMVKSEPTT EVINFWELMS GLDGDTCRFS PIPVKCNGFS 

       130        140        150        160        170        180 
GGLKKENSDP NLKNPNDYEV LKPLDPKLAE ESERLCDGGE NRVVIYTTSL RGVRRTFEAC 

       190        200        210        220        230        240 
NAVRAAIESF GVVVCERDVS MDRGFREELS NLMAVESTAA VLPPRVFVKG KYIGGAEEVM 

       250        260        270        280        290        300 
RLVEEGLLGE LLKEIPRKKD RCGGGCGGCG GLAFLPCSGC NGSCKVVEGW GNDAVVVKCK 

       310 
ECNENGLVRC PICS 

References and documentation are available.

Number of amino acids: 314

Molecular weight: 33615.35

Theoretical pI: 5.37

Amino acid composition: 
Ala (A)  14	  4.5%
Arg (R)  16	  5.1%
Asn (N)  16	  5.1%
Asp (D)  13	  4.1%
Cys (C)  16	  5.1%
Gln (Q)   1	  0.3%
Glu (E)  26	  8.3%
Gly (G)  31	  9.9%
His (H)   2	  0.6%
Ile (I)   9	  2.9%
Leu (L)  28	  8.9%
Lys (K)  18	  5.7%
Met (M)   9	  2.9%
Phe (F)  11	  3.5%
Pro (P)  20	  6.4%
Ser (S)  35	 11.1%
Thr (T)  16	  5.1%
Trp (W)   2	  0.6%
Tyr (Y)   4	  1.3%
Val (V)  27	  8.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1447
Hydrogen    H	      2331
Nitrogen    N	       403
Oxygen      O	       465
Sulfur      S	        25

Formula: C1447H2331N403O465S25
Total number of atoms: 4671

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    17960
Abs 0.1% (=1 g/l)   0.534, assuming all pairs of Cys residues form cystines


Ext. coefficient    16960
Abs 0.1% (=1 g/l)   0.505, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.55
This classifies the protein as unstable.



Aliphatic index: 75.35

Grand average of hydropathicity (GRAVY): -0.197