ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGGEIITLQA GQCGNHVGKF LWSQLAKEHA IGTDGLSQLP DSSTERDDDT KPFFRENSRN 

        70         80         90        100        110        120 
KFTPRAIMMD SEPSVIADVE NTFRGFFDPR NTWVASDGAS AGNSWANGYD IGTRNQDDIL 

       130        140        150        160        170        180 
NKIDKEIDST DNFEGFQLLH SVAGGTGSGL GSNLLEALCD RYPKKILTTY SVFPARSSEV 

       190        200        210        220        230        240 
VVQSYNTILA LRRLIEDSDA TVVFDNASLL NISGKVFRNP NIDLQHTNQL ISTIISSVTN 

       250        260        270        280        290        300 
SIRFPSYMYS SMSSIYSTLI PSPELHFLSP SFTPFTSDYI HDDIAHKGHS SYDVMLDLLD 

       310        320        330        340        350        360 
PSNSLVSTAM NNPTYFNVYN TIIGNVEPRQ ISRAMTKLQQ RIKFPSWSSS AMHVNIGRRS 

       370        380        390        400        410        420 
PYLPLQPNEN EVSGMMLSNM STVVNVFENA CNTFDKVFAK GAFLNNYNVG DLFQSMQNVQ 

       430        440        450        460        470 
DEFAESREVV QSLMEDYVAA EQDSYLDDVL VDDENMVGEL EEDLDADGDH KLV 

References and documentation are available.

Number of amino acids: 473

Molecular weight: 52627.49

Theoretical pI: 4.56

Amino acid composition: 
Ala (A)  26	  5.5%
Arg (R)  19	  4.0%
Asn (N)  36	  7.6%
Asp (D)  39	  8.2%
Cys (C)   3	  0.6%
Gln (Q)  18	  3.8%
Glu (E)  25	  5.3%
Gly (G)  27	  5.7%
His (H)  10	  2.1%
Ile (I)  27	  5.7%
Leu (L)  39	  8.2%
Lys (K)  15	  3.2%
Met (M)  15	  3.2%
Phe (F)  24	  5.1%
Pro (P)  20	  4.2%
Ser (S)  53	 11.2%
Thr (T)  26	  5.5%
Trp (W)   4	  0.8%
Tyr (Y)  15	  3.2%
Val (V)  32	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      2309
Hydrogen    H	      3563
Nitrogen    N	       623
Oxygen      O	       750
Sulfur      S	        18

Formula: C2309H3563N623O750S18
Total number of atoms: 7263

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44475
Abs 0.1% (=1 g/l)   0.845, assuming all pairs of Cys residues form cystines


Ext. coefficient    44350
Abs 0.1% (=1 g/l)   0.843, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.76
This classifies the protein as unstable.



Aliphatic index: 79.53

Grand average of hydropathicity (GRAVY): -0.341