ProtParam
User-provided sequence:
10 20 30 40 50 60
MGGELVTGLG ALRRRKRLLE QEKRVAGWAL VLAGTGIGLM VLHAEMLWFL GCKWVLYLLL
70 80 90 100 110 120
VKCLITLSTA FLLCLIVVFH AKEVQLFMTD NGLRDWRVAL TRRQVAQILL ELLVCGVHPV
130 140 150 160 170 180
PLRSPHCTLA GEATDSQAWP GFLGEGEALL SLAMLLRLYL VPRAVLLRSG VLLNASYRSI
190 200 210 220 230 240
GALNQVRFRH WFVAKLYMNT HPGRLLLGLT LGLWLTTAWV LSVAERQAVN ATGHLTDTLW
250 260 270 280 290 300
LIPITFLTIG YGDVVPGTLW GKIVCLCTGV MGVCCTALLV AVVARKLEFN KAEKHVHNFM
310 320 330 340 350 360
MDIHYAKEMK ESAARLLQEA WMYYKHTRRK DSRAARRHQR KMLAAIHTFR QVRLKHRKLR
370 380 390 400 410 420
EQVNSMVDIS KMHMILCDLQ LGLSASHLAL EKRIDGLAGK LDALTELLST ALQQQPPEPI
QEAT
References and
documentation are available.
Number of amino acids: 424
Molecular weight: 47638.71
Theoretical pI: 9.76
Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 51
Atomic composition:
Carbon C 2157
Hydrogen H 3498
Nitrogen N 604
Oxygen O 559
Sulfur S 25
Formula: C
2157H
3498N
604O
559S
25
Total number of atoms: 6843
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 73045
Abs 0.1% (=1 g/l) 1.533, assuming all pairs of Cys residues form cystines
Ext. coefficient 72420
Abs 0.1% (=1 g/l) 1.520, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.59
This classifies the protein as unstable.
Aliphatic index: 117.31
Grand average of hydropathicity (GRAVY): 0.280