ProtParam
User-provided sequence:
10 20 30 40 50 60
MGGILKNPLA LSPEQLAQQD PETLEEFRRQ VYENTQKNAK LTSHKRNIPG LDNTKEEGEI
70 80 90 100 110 120
IGTSSTFLPK DTLSLKHEQD MLAKMTPEER VQWNQRNLAE NEITKKQFQD IHIDEPKTPY
130 140 150 160 170 180
QGAVDPHGEY YRVDDDEDED NSDKKPCQVA NDDIDDLSLG EPEFEIKENK QPDFETNDEN
190 200 210 220
DEDSPEARHK KFEEMRKKHY DVRAIFNKKS REALKDEDED EDDSTTKEP
References and
documentation are available.
Number of amino acids: 229
Molecular weight: 26648.99
Theoretical pI: 4.57
Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 33
Atomic composition:
Carbon C 1143
Hydrogen H 1788
Nitrogen N 322
Oxygen O 403
Sulfur S 5
Formula: C
1143H
1788N
322O
403S
5
Total number of atoms: 3661
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 0.486, assuming all pairs of Cys residues form cystines
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 0.486, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 54.57
This classifies the protein as unstable.
Aliphatic index: 54.54
Grand average of hydropathicity (GRAVY): -1.441