ProtParam
User-provided sequence:
10 20 30 40 50 60
MGGQESISNN NSDSFIMSSP NLDSQESSIS PIDEKKGTDM QTKSLSSYSK GTLLSKQVQN
70 80 90 100 110 120
LLEEANKYDP IYGSSLPRGF LRDRNTKGKD NGLVPLVEKV IPPIHKKTNN RNTRKKSSTT
130 140 150 160 170 180
TKKDVKKPKA AKVKGKNGRT NHKHTPISKQ EIDTAREKKP LKKGRANKKN DRDSPSSTFV
190 200 210 220 230 240
DWNGPCLRLQ YPLFDIEYLR SHEIYSGTPI QSISLRTNSP QPTSLTSDND TSSVTTAKLQ
250 260 270 280 290 300
SILFSNYMEE YKVDFKRSTA IYNPMSEIGK LIEYSCLVFL PSPYAEQLKE TILPDLNASF
310 320 330 340 350 360
DNSDTKGFVN AINLYNKMIR EIPRQRIIDH LETIDKIPRS FIHDFLHIVY TRSIHPQANK
370 380 390 400 410 420
LKHYKAFSNY VYGELLPNFL SDVYQQCQLK KGDTFMDLGS GVGNCVVQAA LECGCALSFG
430 440 450 460 470 480
CEIMDDASDL TILQYEELKK RCKLYGMRLN NVEFSLKKSF VDNNRVAELI PQCDVILVNN
490 500 510 520 530 540
FLFDEDLNKK VEKILQTAKV GCKIISLKSL RSLTYQINFY NVENIFNRLK VQRYDLKEDS
550 560 570 580
VSWTHSGGEY YISTVMEDVD ESLFSPAARG RRNRGTPVKY TR
References and
documentation are available.
Number of amino acids: 582
Molecular weight: 66201.32
Theoretical pI: 9.15
Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 85
Atomic composition:
Carbon C 2926
Hydrogen H 4666
Nitrogen N 810
Oxygen O 898
Sulfur S 20
Formula: C
2926H
4666N
810O
898S
20
Total number of atoms: 9320
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 48875
Abs 0.1% (=1 g/l) 0.738, assuming all pairs of Cys residues form cystines
Ext. coefficient 48250
Abs 0.1% (=1 g/l) 0.729, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.69
This classifies the protein as unstable.
Aliphatic index: 79.36
Grand average of hydropathicity (GRAVY): -0.614