ProtParam
User-provided sequence:
10 20 30 40 50 60
MGGSSDSDSH DGYLTSEYNS SNSLFSLNTG NSYSSASLDR ATLDCQDSVF FDNHKSSLLS
70 80 90 100 110 120
TEVPRFISND PLHLPITLNY KRDNADPTYT NGKVNKFMIV LIGLPATGKS TISSHLIQCL
130 140 150 160 170 180
KNNPLTNSLR CKVFNAGKIR RQISCATISK PLLLSNTSSE DLFNPKNNDK KETYARITLQ
190 200 210 220 230 240
KLFHEINNDE CDVGIFDATN STIERRRFIF EEVCSFNTDE LSSFNLVPII LQVSCFNRSF
250 260 270 280 290 300
IKYNIHNKSF NEDYLDKPYE LAIKDFAKRL KHYYSQFTPF SLDEFNQIHR YISQHEEIDT
310 320 330 340 350 360
SLFFFNVINA GVVEPHSLNQ SHYPSTCGKQ IRDTIMVIEN FINHYSQMFG FEYIEAVKLF
370 380 390
FESFGNSSEE TLTTLDSVVN DKFFDDLQSL IESNGFA
References and
documentation are available.
Number of amino acids: 397
Molecular weight: 45317.63
Theoretical pI: 5.39
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 2020
Hydrogen H 3085
Nitrogen N 533
Oxygen O 631
Sulfur S 12
Formula: C
2020H
3085N
533O
631S
12
Total number of atoms: 6281
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 22850
Abs 0.1% (=1 g/l) 0.504, assuming all pairs of Cys residues form cystines
Ext. coefficient 22350
Abs 0.1% (=1 g/l) 0.493, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.87
This classifies the protein as unstable.
Aliphatic index: 78.82
Grand average of hydropathicity (GRAVY): -0.385