ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGGSSGGGVS YRSGGESDVE LEDYEVDDFR DGIVESRGNR FNPLTNFLGL DFAGGSGGKF 

        70         80         90        100        110        120 
TVINGIRDIS RGSIVHPDNR WYKAWTMFIL IWALYSSFFT PLEFGFFRGL PENLFILDIA 

       130        140        150        160        170        180 
GQIAFLVDIV LTFFVAYRDS RTYRMIYKRS SIALRYLKST FIIDLLACMP WDIIYKAAGE 

       190        200        210        220        230        240 
KEEVRYLLLI RLYRVHRVIL FFHKMEKDIR INYLFTRIVK LIFVELYCTH TAACIFYYLA 

       250        260        270        280        290        300 
TTLPASQEGY TWIGSLKLGD YSYSKFREID LWTRYTTSMY FAVVTMATVG YGDIHAVNMR 

       310        320        330        340        350        360 
EMIFAMVYIS FDMILGAYLI GNMTALIVKG SKTERFRDKM ADIMRYMNRN KLGRNIRGQI 

       370        380        390        400        410        420 
TGHLRLQYES SYTEAAVLQD IPVSIRAKIA QTLYLPYIEK VPLFRGCSSE FINQIVIRLH 

       430        440        450        460        470        480 
EEFFLPGEVI MEQGSVVDQL YFVCHGVLEE IGITKDGSEE IVAVLQPDHS FGEISILCNI 

       490        500        510        520        530        540 
PQPYTVRVAE LCRILRLDKQ SFMNILEIFF HDGRRILNNL LEGKESNVRI KQLESDITFH 

       550        560        570        580        590        600 
ISKQEAELAL KLNSAAFYGD LYQLKSLIRA GGDPNKTDYD GRSPLHLAAS RGYEDITLYL 

       610        620        630        640        650        660 
IQESVDVNIK DKLGSTPLLE AIKNGNDRVA ALLVKEGATL NIENAGTFLC TVVAKGDSDF 

       670        680        690        700        710        720 
LKRLLSNGID PNSKDYDHRT PLHVAASEGF YVLAIQLVEA SANVLAKDRW GNTPLDEALG 

       730        740        750        760        770        780 
CGNKMLIKLL EDAKNSQISS FPSGSKEPKD KVYKKKCTVY FSHPGDSKEK RRRGIVLWVP 

       790        800        810        820 
RSIEELIRTA KEQLNVPEAS CVLSEDEAKI IDVDLISDGQ KLYLAVET 

References and documentation are available.

Number of amino acids: 828

Molecular weight: 93899.23

Theoretical pI: 6.27

Amino acid composition: 
Ala (A)  52	  6.3%
Arg (R)  50	  6.0%
Asn (N)  32	  3.9%
Asp (D)  49	  5.9%
Cys (C)  11	  1.3%
Gln (Q)  20	  2.4%
Glu (E)  54	  6.5%
Gly (G)  59	  7.1%
His (H)  15	  1.8%
Ile (I)  72	  8.7%
Leu (L)  89	 10.7%
Lys (K)  47	  5.7%
Met (M)  18	  2.2%
Phe (F)  42	  5.1%
Pro (P)  24	  2.9%
Ser (S)  57	  6.9%
Thr (T)  37	  4.5%
Trp (W)   8	  1.0%
Tyr (Y)  40	  4.8%
Val (V)  52	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 103
Total number of positively charged residues (Arg + Lys): 97

Atomic composition:

Carbon      C	      4254
Hydrogen    H	      6669
Nitrogen    N	      1115
Oxygen      O	      1221
Sulfur      S	        29

Formula: C4254H6669N1115O1221S29
Total number of atoms: 13288

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   104225
Abs 0.1% (=1 g/l)   1.110, assuming all pairs of Cys residues form cystines


Ext. coefficient   103600
Abs 0.1% (=1 g/l)   1.103, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.15
This classifies the protein as unstable.



Aliphatic index: 100.33

Grand average of hydropathicity (GRAVY): -0.046