ProtParam
User-provided sequence:
10 20 30 40 50 60
MGGVFSGDRV FNDSKAIHNV DKQMLHNHDP TLSVSTPEVT TEMHERLNFP KVELHLHLDG
70 80 90 100 110 120
AVRFDTLIDL SQQKGIPLAG AKTVEELKKV LVTHEPANLS KVLEAFEIFL PVIRGDLAAI
130 140 150 160 170 180
ERVAYELCED QHNNGVVYFE GRYSPHLLLC NDYPEVTAAH VVAAVKKGFD RGEKQFGIKA
190 200 210 220 230 240
RSILCCIRGL DKKFPQLILD LATDLKQLGV VAIDVAGSAH GADEQYEPEV VAAFQEAHKR
250 260 270 280 290 300
GIHRTVHAGE SGGPKEVIKA IEDMYAERIG HGYRVMRDEE MYLEHFVNSK NVHLEACPYS
310 320 330 340 350 360
SVMTGAVPLD WKNHPIARWA KDDVNFSVSR DDPTCFDNSM LSELTLVHKQ IGLDVHQLWK
370 380 390
AQLNAARSCF LPEDEKAELV KRVEAGEPPR P
References and
documentation are available.
Number of amino acids: 391
Molecular weight: 43675.76
Theoretical pI: 5.73
Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 44
Atomic composition:
Carbon C 1941
Hydrogen H 3050
Nitrogen N 542
Oxygen O 576
Sulfur S 15
Formula: C
1941H
3050N
542O
576S
15
Total number of atoms: 6124
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 30285
Abs 0.1% (=1 g/l) 0.693, assuming all pairs of Cys residues form cystines
Ext. coefficient 29910
Abs 0.1% (=1 g/l) 0.685, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.44
This classifies the protein as stable.
Aliphatic index: 90.00
Grand average of hydropathicity (GRAVY): -0.293