ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGHHSCCNKQ KVKRGLWSPE EDEKLINYIN SYGHGCWSSV PKHAGTYTHI HGFCLQRCGK 

        70         80         90        100        110        120 
SCRLRWINYL RPDLKRGSFS PQEAALIIEL HSILGNRWAQ IAKHLPGRTD NEVKNFWNSS 

       130        140        150        160        170        180 
IKKKLMSHHH HGHHHHHLSS MASLLTNLPY HNGFNPTTVD DESSRFMSNI ITNTNPNFIT 

       190        200        210        220        230        240 
PSHLSLPSPH VMTPLMFPTS REGDFKFLTT NNPNQSHHHD NNHYNNLDIL SPTPTINNHH 

       250        260        270        280        290        300 
QPSLSSCPHD NNLQWPALPD FPASTISGFQ ETLQDYDDAN KLNVFVTPFN DNAKKLLCGE 

       310        320        330        340        350        360 
VLEGKVLSSS SPISQDHGLF LPTTYNFQMT STSDHQHHHR VDSYINHMII PSSSSSSPIS 


CGQYVIT 

References and documentation are available.

Number of amino acids: 367

Molecular weight: 41576.49

Theoretical pI: 7.46

Amino acid composition: 
Ala (A)  10	  2.7%
Arg (R)  11	  3.0%
Asn (N)  32	  8.7%
Asp (D)  17	  4.6%
Cys (C)   9	  2.5%
Gln (Q)  13	  3.5%
Glu (E)  11	  3.0%
Gly (G)  18	  4.9%
His (H)  33	  9.0%
Ile (I)  21	  5.7%
Leu (L)  32	  8.7%
Lys (K)  17	  4.6%
Met (M)   8	  2.2%
Phe (F)  15	  4.1%
Pro (P)  26	  7.1%
Ser (S)  44	 12.0%
Thr (T)  23	  6.3%
Trp (W)   6	  1.6%
Tyr (Y)  10	  2.7%
Val (V)  11	  3.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 28

Atomic composition:

Carbon      C	      1833
Hydrogen    H	      2778
Nitrogen    N	       534
Oxygen      O	       546
Sulfur      S	        17

Formula: C1833H2778N534O546S17
Total number of atoms: 5708

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48400
Abs 0.1% (=1 g/l)   1.164, assuming all pairs of Cys residues form cystines


Ext. coefficient    47900
Abs 0.1% (=1 g/l)   1.152, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 56.27
This classifies the protein as unstable.



Aliphatic index: 67.74

Grand average of hydropathicity (GRAVY): -0.641