ProtParam
User-provided sequence:
10 20 30 40 50 60
MGHLKSLFTI LFLIAMSSTV TFAGKIPAII VFGDSSVDAG NNNYIPTVAR SNFEPYGRDF
70 80 90 100 110 120
VGGKPTGRFC NGKIATDFMS EALGLKPIIP AYLDPSYNIS DFATGVTFAS AATGYDNATS
130 140 150 160 170 180
DVLSVLPLWK QLEYYKEYQT KLKAYQGKDR GTETIESSLY LISIGTNDFL ENYFAFPGRS
190 200 210 220 230 240
SQYSVSLYQD FLAGIAKEFV KKLHGLGARK ISLGGLPPMG CMPLERATNI GTGGECVGRY
250 260 270 280 290 300
NDIAVQFNSK LDKMVEKLSK ELPGSNLVFS NPYEPFMRII KNPSSFGFEV VGAACCATGM
310 320 330 340 350
FEMGYGCQRN NPFTCTNADK YVFWDSFHPT QKTNHIMANA LMNSTFPHFL
References and
documentation are available.
Number of amino acids: 350
Molecular weight: 38477.97
Theoretical pI: 7.55
Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 31
Atomic composition:
Carbon C 1742
Hydrogen H 2654
Nitrogen N 442
Oxygen O 507
Sulfur S 18
Formula: C
1742H
2654N
442O
507S
18
Total number of atoms: 5363
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36705
Abs 0.1% (=1 g/l) 0.954, assuming all pairs of Cys residues form cystines
Ext. coefficient 36330
Abs 0.1% (=1 g/l) 0.944, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 28.56
This classifies the protein as stable.
Aliphatic index: 75.00
Grand average of hydropathicity (GRAVY): -0.081