ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGHSGREERI KDIFKELTSK ELTPGLLLTL QKLAQKPNTN LEQFIASCKA LTKLSSNNPI 

        70         80         90        100        110        120 
IFNELLELLK NKSEEDSTGP KKIAPSINKR KKFKIQLDLD DNEDELDSPV QKKPAPTRTL 

       130        140        150        160        170        180 
FKRIDKLKAK QLRQYSPTVK DPSPNSEQQT QNGHAETKDY EPTRSEVVEE DREWYDNDDD 

       190        200        210        220        230        240 
YGNLVPEPLS ELPEEAKLLP VIRNIDNDDA LRNTVQLYPI PLKQRMEWIP PFLSKFALEN 

       250        260        270        280        290        300 
KVPTSIIIGS ISETSSQVSA LSMVNPFRNP DSEFSANAKR GSKLVALRRI NMEHIQQSRD 

       310        320        330        340        350        360 
NTTVLNTAMG EVLGLENNNK AKDKSNQKIC DDTALFTPSK DDIKHTKEQL PVFRCRSQLL 

       370        380        390        400        410        420 
SLIRENQVVV IIGETGSGKT TQLAQYLYEE GYANDRGKSI VVTQPRRVAA ISVAKRVAME 

       430        440        450        460        470        480 
MQVPLGKEVG YSIRFEDVTD SECTKLKFVT DGILLRETLL DDTLDKYSCV IIDEAHERSL 

       490        500        510        520        530        540 
NTDILLGFFK ILLARRRDLK LIITSATMNA KKFSAFFGNA PQFTIPGRTF PVQTIYTSNP 

       550        560        570        580        590        600 
VQDYVEAAVS QAVKIHLAND CSSGDILIFM TGQEDIETTF DTLQEKFLQV YSKKFGTANF 

       610        620        630        640        650        660 
EEINDIEILP IYSALPADLQ FKIFQDLHGT KRKIIIATNI AETSLTIKGI RYVIDCGYSK 

       670        680        690        700        710        720 
LKVYNPKIGL DSLVITPISK ANADQRSGRA GRTAPGTAYR LYTEDTFKED MYLQTIPEIQ 

       730        740        750        760        770        780 
RTNLSNTLLL LKSLDVTDEL SKFPFIDKPP LQTFLSSLYE LWFIGAIDTS GQLTPLGLQM 

       790        800        810        820        830        840 
AKFPLQPSLS KILLIAVRNG CSDEMLTIVS MLSVPQVFYR PKERQKEADI ARNKFFIAKS 

       850        860        870        880        890        900 
DHLTLLNVFE QWRANNFSSH WCNKHFVQYK SLVRARDIRD QLLTILKSQK IPVISSGKDW 

       910        920        930        940        950        960 
DIIKKCICSG FAHQAAKITG LRNYVHLKTG VSVQLHPTSA LHGLGDLPPY VVYHELLMTS 

       970        980        990       1000       1010       1020 
KEYICCVTSV DPFWLMEYGG LLYDIKRIKN DQEATTTGLF GEHYEHTLDK VEDDIDINIR 

      1030       1040       1050       1060       1070 
RCKDMRDSVI QELKMTDNSN KEDKKQKTKK QNILNGKENS MKPFKRRKPF F 

References and documentation are available.

Number of amino acids: 1071

Molecular weight: 121652.54

Theoretical pI: 8.42

Amino acid composition: 
Ala (A)  56	  5.2%
Arg (R)  52	  4.9%
Asn (N)  54	  5.0%
Asp (D)  71	  6.6%
Cys (C)  14	  1.3%
Gln (Q)  51	  4.8%
Glu (E)  68	  6.3%
Gly (G)  46	  4.3%
His (H)  17	  1.6%
Ile (I)  81	  7.6%
Leu (L) 113	 10.6%
Lys (K)  94	  8.8%
Met (M)  18	  1.7%
Phe (F)  45	  4.2%
Pro (P)  52	  4.9%
Ser (S)  76	  7.1%
Thr (T)  72	  6.7%
Trp (W)   7	  0.7%
Tyr (Y)  30	  2.8%
Val (V)  54	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 139
Total number of positively charged residues (Arg + Lys): 146

Atomic composition:

Carbon      C	      5427
Hydrogen    H	      8699
Nitrogen    N	      1467
Oxygen      O	      1633
Sulfur      S	        32

Formula: C5427H8699N1467O1633S32
Total number of atoms: 17258

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    84075
Abs 0.1% (=1 g/l)   0.691, assuming all pairs of Cys residues form cystines


Ext. coefficient    83200
Abs 0.1% (=1 g/l)   0.684, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.10
This classifies the protein as stable.



Aliphatic index: 90.49

Grand average of hydropathicity (GRAVY): -0.421