ProtParam
User-provided sequence:
10 20 30 40 50 60
MGIFRWNYPE SSVPGVWGET TSTIDWCEEN YVVSPYIAEW SNTLTNSVFI LSAIYTTYSA
70 80 90 100 110 120
YKNKLEKRFL LIGFGYGLVG VGSWLFHMTL KYRFQLLDEL PMIYAMCIPT WSLVCEAKEA
130 140 150 160 170 180
LLNGDNHKKV PLFEQIFIGV IIGLAVTTAS ILYVIYKNVD IHQILFGVQI VVVAATAGSL
190 200 210 220 230 240
TYRYVHDPLA KRNLKASMAL GAILFLSGYI SWLLDIHYCS FWVHVRRSIL ALPLGVLLEP
250 260 270 280 290 300
HGWWHILTGM GIYFYIVSLE HLRVITLNVS CNYQFIWRWK VFPELIWKGR KPSTRYSLEL
310
FGPYVEDQSI EVKKEK
References and
documentation are available.
Number of amino acids: 316
Molecular weight: 36419.69
Theoretical pI: 8.44
Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 27
Atomic composition:
Carbon C 1717
Hydrogen H 2599
Nitrogen N 413
Oxygen O 440
Sulfur S 11
Formula: C
1717H
2599N
413O
440S
11
Total number of atoms: 5180
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 101550
Abs 0.1% (=1 g/l) 2.788, assuming all pairs of Cys residues form cystines
Ext. coefficient 101300
Abs 0.1% (=1 g/l) 2.781, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.80
This classifies the protein as stable.
Aliphatic index: 111.30
Grand average of hydropathicity (GRAVY): 0.317