ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGIITLSGNV LHLLKAYPKK GLEEVSQPEP NTANDSSTEY KGKSKDDFQM VEKSNTDERY 

        70         80         90        100        110        120 
NFTRTKKWFL LMTSEYYKLM ENRLLMFCII ACSFICAIQF LFFIIYWTNI VPRKTQRAIT 

       130        140 
NLNYDYLTAH LKEQCVPYAK ILDQCIL 

References and documentation are available.

Number of amino acids: 147

Molecular weight: 17262.12

Theoretical pI: 8.54

Amino acid composition: 
Ala (A)   7	  4.8%
Arg (R)   5	  3.4%
Asn (N)   9	  6.1%
Asp (D)   6	  4.1%
Cys (C)   5	  3.4%
Gln (Q)   6	  4.1%
Glu (E)   9	  6.1%
Gly (G)   4	  2.7%
His (H)   2	  1.4%
Ile (I)  12	  8.2%
Leu (L)  16	 10.9%
Lys (K)  13	  8.8%
Met (M)   5	  3.4%
Phe (F)   8	  5.4%
Pro (P)   5	  3.4%
Ser (S)   8	  5.4%
Thr (T)  11	  7.5%
Trp (W)   2	  1.4%
Tyr (Y)   9	  6.1%
Val (V)   5	  3.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 15
Total number of positively charged residues (Arg + Lys): 18

Atomic composition:

Carbon      C	       785
Hydrogen    H	      1222
Nitrogen    N	       196
Oxygen      O	       221
Sulfur      S	        10

Formula: C785H1222N196O221S10
Total number of atoms: 2434

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24660
Abs 0.1% (=1 g/l)   1.429, assuming all pairs of Cys residues form cystines


Ext. coefficient    24410
Abs 0.1% (=1 g/l)   1.414, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 23.33
This classifies the protein as stable.



Aliphatic index: 88.91

Grand average of hydropathicity (GRAVY): -0.197