ProtParam
User-provided sequence:
10 20 30 40 50 60
MGIKDLLRFM KPYILPIHIQ KYAGKRVGID AYSWLHKGAY SCSMELCLDT DGKKKLRYID
70 80 90 100 110 120
YFMHRVNLLQ HYEIIPVVVL DGGNMPCKAA TGDERHRKRK ANFDAAMVKL KEGNVAAATE
130 140 150 160 170 180
LFQRAVSVTS SMAHQLIQVL KSENVEFIVA PYEADAQLAY LSSLELEQGG IAAVITEDSD
190 200 210 220 230 240
LLAYGCKAVI FKMDRYGKGE ELVLDNVFQA VDQKPSFQNF DQELFTAMCV LAGCDFLPSV
250 260 270 280 290 300
PGVGISRAHA FISKYQSVEL VLSFLKTKKG KLVPDDYSSS FTEAVSVFQH ARVYDFDAKK
310 320 330 340 350 360
LKHLKPLSHN LLNLPVEQLE FLGPDLSPSV AVAIAEGNVD PITMKAFNHF SVSRKPLKTP
370 380 390 400 410 420
VRSFKEQEKG SSFLVCSLSK SEEIIELKRT ADEAMIDPEA IVKKKMYSKQ DSDLYKLLAQ
430 440 450 460 470 480
PNRDQVVTRP SNPSLIPDNN PFKIRKTDEI NLEIAEYGVQ ELADSFVTKS KAVDVASSSP
490 500 510 520 530 540
NSHEHEDQKE CVTIDLSKLD AAGIKQDSEK NRVKETFETT EVDLAEVQDH VNMTTKRVRG
550 560 570
TKPRTENFKV KTSCKSSENN KTIIKKKHSI LDFFQRL
References and
documentation are available.
Number of amino acids: 577
Molecular weight: 64778.36
Theoretical pI: 7.58
Total number of negatively charged residues (Asp + Glu): 78
Total number of positively charged residues (Arg + Lys): 79
Atomic composition:
Carbon C 2888
Hydrogen H 4608
Nitrogen N 776
Oxygen O 867
Sulfur S 22
Formula: C
2888H
4608N
776O
867S
22
Total number of atoms: 9161
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 31330
Abs 0.1% (=1 g/l) 0.484, assuming all pairs of Cys residues form cystines
Ext. coefficient 30830
Abs 0.1% (=1 g/l) 0.476, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.28
This classifies the protein as stable.
Aliphatic index: 87.35
Grand average of hydropathicity (GRAVY): -0.328