ProtParam
User-provided sequence:
10 20 30 40 50 60
MGILDSLKKP VNIKSSLPKF SRSATSINLS SHPVSSRSFL ELPPELFSIT KPIFKLLQAH
70 80 90 100 110 120
ANKIYFQSSD AEAVWNVKDS SGHVFEAESI SLLGSHIIIT NSSVQVADVA IIDSPSNINQ
130 140 150 160 170 180
CEISSVGEFL QFNNGQLSIT CNDFGLLEKF KRLCMISIFE FISIYKALTG TVISSYGLRM
190 200 210 220 230 240
SDMHIILNSP FNFKDWCEVY LDGQGWVKVW CHIDKVSKTN NSKSSSDNDA KGKYQIRFYR
250 260 270 280 290 300
DDKSTSSKNC VFFIPDNEYV QDIFFYNINA AEPSKNMNDF FQGLQMIKLV GNVRFCSDTD
310 320
FNDVVDSGSI YSSANNGSVT AARQH
References and
documentation are available.
Number of amino acids: 325
Molecular weight: 36361.14
Theoretical pI: 6.17
Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 30
Atomic composition:
Carbon C 1626
Hydrogen H 2508
Nitrogen N 426
Oxygen O 495
Sulfur S 13
Formula: C
1626H
2508N
426O
495S
13
Total number of atoms: 5068
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 35785
Abs 0.1% (=1 g/l) 0.984, assuming all pairs of Cys residues form cystines
Ext. coefficient 35410
Abs 0.1% (=1 g/l) 0.974, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.54
This classifies the protein as unstable.
Aliphatic index: 84.55
Grand average of hydropathicity (GRAVY): -0.149