ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGILLPPVSQ VTNNHINHTQ HHRNRSLDSA LQRIPEVEVS SPNAESENTF CSPSILGATM 

        70         80         90        100        110        120 
CSKIATTAAT VTSALSPPGT PTPALMSTAC TPTIGAVKGN VQHPVPKHEQ SISLAAACRS 

       130        140        150        160        170        180 
SAVVESCPTL RMRPKSSESV AANGQEMIGV IVSSTGNGSS SSNNSCSSAG STKKREDLTS 

       190        200        210        220        230        240 
LGSDDSGIIC GSESDQISLN RICHSHESLD SGEMDADADA EEECVDLMDT TSIDEEYNIM 

       250        260        270        280        290        300 
QPDHLCLYPP QAASTELDCR TLRPSRKQKR QQQQVLERKN LPSQNQIESE DSVDAGVDAR 

       310        320        330        340        350        360 
TRYRVMNMSQ AAINVELGTA SNEIDPDVNY RESHQDPIAD APSLSTPSVA STASATPSVT 

       370        380        390        400        410        420 
PTPIATPTPT ESTKNDQVLF KNFFGATKNA IFRTAQSIIE NHEKKNAAKQ KEQPDHSGTV 

       430        440        450        460        470        480 
SLVGSPNGGS SVPQDLVKSP TDISKKKEFF SLLTSSSSIK AAAAAAANSA ASTPPATPTE 

       490        500        510        520        530        540 
ALAANSNSKL QPLSDPSADL KVKERTLNLR LPGDSEGSEG ALTKCSSINS IHKLKLPVPG 

       550        560        570        580        590        600 
VVKYFISEKA PVSDVLQKPE KGQSGLLRFF ESPVFNIHFA VHYLFYSKEP GVLSFIGNKI 

       610        620        630        640        650        660 
FSFPDQEVDL YIPQLVVMYI QMDELAEVLD PYLTIRCRKS VDFSLKCLWL LEAYNYQVDS 

       670        680        690        700        710        720 
LGNSHNSSRK SKLALMKEIF SKREHKQTQN DLKSAGTGGL HVGEPRSVVA LAKKTHHRSQ 

       730        740        750        760        770        780 
SDATVLLADF RSPHTLSISH RMYQQPPYTT QTLPTTPAKL CLGDLTSGHA FDNGCTCFET 

       790        800        810        820        830        840 
VRGQVNGLLG QRTLCSCGAP KTSPQKEFMK ALMNVGKNLT SWPSKAEKTS ALRMSLNLIN 

       850        860        870        880        890        900 
KNLPARVWLP LYSDIPHHVV RITEEKTAVL NSKDKTPYII YVEVVEIPDI YTSPLIPKMM 

       910        920        930        940        950        960 
PSLRHTKSEE HLDGSCLNSH QHLSCSRTSS CSSSQQGTSC RRKKGADVDG GCGDAGPHNS 

       970        980        990       1000       1010       1020 
CSNVHSLGGL QFQEDDVWSQ EEDEITAQYL NMRKVSERDT ISQISLDSCD SRDQGPPVVF 

      1030       1040       1050       1060       1070       1080 
NIGDVRLRHC SNLSCENTKS FSNDPEDPSA AALKEPWHEK EKLIRESSPY GHLSNWRLLS 

      1090       1100       1110       1120       1130       1140 
AIVKCGDDLR QELMATQLLQ MFKIIWQEEQ VDLWVRPYKI VCLSNDSGLI EPILNTVSLH 

      1150       1160       1170       1180       1190       1200 
QIKKNSNKSL KEYFIDEYGS PSGESFRRAQ KNFVQSCAAY CLISYLLQVK DRHNGNILFH 

      1210       1220       1230       1240       1250       1260 
SDGHIIHIDF GFILSISPKN LGFEQSPFKL TPEFVEVMGG TSSEHWREFN KLLLVGMMSA 

      1270       1280       1290       1300       1310       1320 
RKHMDRIINF VEIMRSNAHL PCFKNGCSGT VQNLRKRFHM NLTEQEMERK VEQLVQDSLK 

      1330 
SLSTKLYDGY QYYTNGIL 

References and documentation are available.

Number of amino acids: 1338

Molecular weight: 147244.18

Theoretical pI: 6.48

Amino acid composition: 
Ala (A)  84	  6.3%
Arg (R)  57	  4.3%
Asn (N)  68	  5.1%
Asp (D)  68	  5.1%
Cys (C)  34	  2.5%
Gln (Q)  63	  4.7%
Glu (E)  81	  6.1%
Gly (G)  69	  5.2%
His (H)  43	  3.2%
Ile (I)  68	  5.1%
Leu (L) 120	  9.0%
Lys (K)  81	  6.1%
Met (M)  29	  2.2%
Phe (F)  39	  2.9%
Pro (P)  78	  5.8%
Ser (S) 163	 12.2%
Thr (T)  78	  5.8%
Trp (W)   9	  0.7%
Tyr (Y)  29	  2.2%
Val (V)  77	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 149
Total number of positively charged residues (Arg + Lys): 138

Atomic composition:

Carbon      C	      6402
Hydrogen    H	     10206
Nitrogen    N	      1816
Oxygen      O	      2038
Sulfur      S	        63

Formula: C6402H10206N1816O2038S63
Total number of atoms: 20525

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    94835
Abs 0.1% (=1 g/l)   0.644, assuming all pairs of Cys residues form cystines


Ext. coefficient    92710
Abs 0.1% (=1 g/l)   0.630, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.51
This classifies the protein as unstable.



Aliphatic index: 77.77

Grand average of hydropathicity (GRAVY): -0.439