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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGILSLITGK AGASGFGSGS TAEQVTAGVD ATGLTVIVTG GASGIGLETS RVFAMRGAHV

70 80 90 100 110 120
IIAARNTEAA SVVRKKIIEE NPKAHIDVLK LDLSSLKSVR AFADQFNSMN LPLNILINNA

130 140 150 160 170 180
GVMFCPFGLS EDGVEMQFAT NHLGHFLLTN LLLDNMKATA KSTGIEGRIV NLSSVAHLHT

190 200 210 220 230 240
YPKGIEFDKL NDEKTYDDKM AYGQSKLANI LHAKELSRRL KEEGANITIN CVHPGLIMTN

250 260 270 280 290 300
LMRHSFFLMR VLQFATYILW KSVPQGAATT CYVGLNPQLK GVTGQYFADC NVEKTSRFAR

310 320
NDALAKQLWE FSEKLIKSSS K


References and documentation are available.

Number of amino acids: 321 Molecular weight: 34841.20 Theoretical pI: 9.06
Amino acid composition: 
Ala (A) 31 9.7% Arg (R) 12 3.7% Asn (N) 19 5.9% Asp (D) 12 3.7% Cys (C) 4 1.2% Gln (Q) 9 2.8% Glu (E) 16 5.0% Gly (G) 27 8.4% His (H) 9 2.8% Ile (I) 20 6.2% Leu (L) 35 10.9% Lys (K) 22 6.9% Met (M) 10 3.1% Phe (F) 15 4.7% Pro (P) 7 2.2% Ser (S) 24 7.5% Thr (T) 21 6.5% Trp (W) 2 0.6% Tyr (Y) 6 1.9% Val (V) 20 6.2% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 28 Total number of positively charged residues (Arg + Lys): 34 Atomic composition: Carbon C 1548 Hydrogen H 2487 Nitrogen N 427 Oxygen O 457 Sulfur S 14 Formula: C1548H2487N427O457S14 Total number of atoms: 4933 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 20190 Abs 0.1% (=1 g/l) 0.579, assuming all pairs of Cys residues form cystines Ext. coefficient 19940 Abs 0.1% (=1 g/l) 0.572, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 29.22 This classifies the protein as stable. Aliphatic index: 94.55 Grand average of hydropathicity (GRAVY): 0.012