ProtParam
User-provided sequence:
10 20 30 40 50 60
MGILSLITGK AGASGFGSGS TAEQVTAGVD ATGLTVIVTG GASGIGLETS RVFAMRGAHV
70 80 90 100 110 120
IIAARNTEAA SVVRKKIIEE NPKAHIDVLK LDLSSLKSVR AFADQFNSMN LPLNILINNA
130 140 150 160 170 180
GVMFCPFGLS EDGVEMQFAT NHLGHFLLTN LLLDNMKATA KSTGIEGRIV NLSSVAHLHT
190 200 210 220 230 240
YPKGIEFDKL NDEKTYDDKM AYGQSKLANI LHAKELSRRL KEEGANITIN CVHPGLIMTN
250 260 270 280 290 300
LMRHSFFLMR VLQFATYILW KSVPQGAATT CYVGLNPQLK GVTGQYFADC NVEKTSRFAR
310 320
NDALAKQLWE FSEKLIKSSS K
References and
documentation are available.
Number of amino acids: 321
Molecular weight: 34841.20
Theoretical pI: 9.06
Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 34
Atomic composition:
Carbon C 1548
Hydrogen H 2487
Nitrogen N 427
Oxygen O 457
Sulfur S 14
Formula: C
1548H
2487N
427O
457S
14
Total number of atoms: 4933
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 20190
Abs 0.1% (=1 g/l) 0.579, assuming all pairs of Cys residues form cystines
Ext. coefficient 19940
Abs 0.1% (=1 g/l) 0.572, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 29.22
This classifies the protein as stable.
Aliphatic index: 94.55
Grand average of hydropathicity (GRAVY): 0.012