ProtParam
User-provided sequence:
10 20 30 40 50 60
MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET
70 80 90 100 110 120
TSHLMGMFYR TIRMMENGIK PVYIFDGKPP QLKSAQLAKR SERRAEAEKQ LQQAQEAGAE
130 140 150 160 170 180
EEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALAKAG KVYAAATEDM
190 200 210 220 230 240
DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RVLQELGLNQ EQFVDLCILL GSDYCESVRG
250 260 270 280 290 300
IGPKRAVDLI QKHKSIEEIV RRLDPSKYPV PENWLHKEAR QLFLEPEVVD PESVELKWSE
310 320 330 340 350 360
PNEEELVKFM CGEKQFSEER IRSGVKRLNK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP
370 380
KGPAKKKAKT GAAGKFRRGK
References and
documentation are available.
Number of amino acids: 380
Molecular weight: 42621.98
Theoretical pI: 8.80
Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 61
Atomic composition:
Carbon C 1878
Hydrogen H 3039
Nitrogen N 533
Oxygen O 564
Sulfur S 16
Formula: C
1878H
3039N
533O
564S
16
Total number of atoms: 6030
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23295
Abs 0.1% (=1 g/l) 0.547, assuming all pairs of Cys residues form cystines
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.538, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 53.61
This classifies the protein as unstable.
Aliphatic index: 79.87
Grand average of hydropathicity (GRAVY): -0.542