ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG GNKKYRALRI 

        70         80         90        100        110        120 
ETGNFSWASE GISKKTRIAG VVYHPSNNEL VRTNTLTKAA IVQIDATPFR QWFEAHYGQT 

       130        140        150        160        170        180 
LGKKKNVKEE ETVAKSKNAE RKWAARAASA KIESSVESQF SAGRLYACIS SRPGQSGRCD 

       190        200 
GYILEGEELA FYLRRLTAKK 

References and documentation are available.

Number of amino acids: 200

Molecular weight: 22489.67

Theoretical pI: 10.67

Amino acid composition: 
Ala (A)  23	 11.5%
Arg (R)  22	 11.0%
Asn (N)   8	  4.0%
Asp (D)   3	  1.5%
Cys (C)   2	  1.0%
Gln (Q)   7	  3.5%
Glu (E)  14	  7.0%
Gly (G)  16	  8.0%
His (H)   4	  2.0%
Ile (I)  11	  5.5%
Leu (L)  10	  5.0%
Lys (K)  22	 11.0%
Met (M)   1	  0.5%
Phe (F)   7	  3.5%
Pro (P)   4	  2.0%
Ser (S)  17	  8.5%
Thr (T)  12	  6.0%
Trp (W)   3	  1.5%
Tyr (Y)   6	  3.0%
Val (V)   8	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 17
Total number of positively charged residues (Arg + Lys): 44

Atomic composition:

Carbon      C	       984
Hydrogen    H	      1604
Nitrogen    N	       314
Oxygen      O	       285
Sulfur      S	         3

Formula: C984H1604N314O285S3
Total number of atoms: 3190

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    25565
Abs 0.1% (=1 g/l)   1.137, assuming all pairs of Cys residues form cystines


Ext. coefficient    25440
Abs 0.1% (=1 g/l)   1.131, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.34
This classifies the protein as unstable.



Aliphatic index: 64.05

Grand average of hydropathicity (GRAVY): -0.830