ProtParam
User-provided sequence:
10 20 30 40 50 60
MGIVQIGHCG CVGAIAAPRN VVKPRKEESR DFSGARGSNK DKSMPWKKLD ANEFGIQRSM
70 80 90 100 110 120
IPDSTRMVLN KLKKKGFQVY LVGGCVRDLI LDRIPKDFDV ITTAELKEVR KVFPGCQIVG
130 140 150 160 170 180
RRFPICHVYV DDIIIEVSSF STSARTGKAP NKSFRRPAGC DERDYIRWKN CLQRDFTVNG
190 200 210 220 230 240
LMFDPSENVV YDYIGGVEDL RNSKVRTVSA ANLSFVEDTA RILRAIRIAA RLGFSLTKDV
250 260 270 280 290 300
AISVKELSSS LLRLDPSRIR MEINYMLAYG SAEASLRLLW RFGLMEILLP IQASYLVSQG
310 320 330 340 350 360
FRRRDGRSNM LLSLFRNLDR LVAPDRPCSE FLWIGILAFH KALVDQPRDP TVVASFCLAI
370 380 390 400 410 420
YSEVSLSEAI AIARSNSKQH NSHFQELSSP EKDTADSESK ISQQVIKLAE SIRSAARKLN
430 440 450 460 470 480
NRDYIANAMS KYPQAPGSDM VFLSRLMLER VEKMFGNVRR KGNQERDDVP SLERRINYKS
490 500
LALGDFHETR RVFARIVFDT IYPLA
References and
documentation are available.
Number of amino acids: 505
Molecular weight: 57150.97
Theoretical pI: 9.63
Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 79
Atomic composition:
Carbon C 2528
Hydrogen H 4075
Nitrogen N 735
Oxygen O 730
Sulfur S 22
Formula: C
2528H
4075N
735O
730S
22
Total number of atoms: 8090
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41870
Abs 0.1% (=1 g/l) 0.733, assuming all pairs of Cys residues form cystines
Ext. coefficient 41370
Abs 0.1% (=1 g/l) 0.724, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.71
This classifies the protein as unstable.
Aliphatic index: 91.33
Grand average of hydropathicity (GRAVY): -0.245