ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGIVTTKTKT YTTTNFDFSN KGFGVGGLKQ FVYGNTFIVL DAFWVSFSNL FRVNPDKETS 

        70         80         90        100        110        120 
LVLFEKQNTK DSSVFGDHQD QNQICGTQDD DQMESSLVSI SKRCEFYSDN KSIVGFVEEP 

       130        140        150        160        170        180 
KAFSFKFHEY SAITVQEEEK KKMVSFSDEK TSSMVLEKSE SDGFMKEHVK EEMLVYEFMS 

       190        200        210        220        230        240 
CGVLKESLVH ENLVGGEDNL FDDDDGFIEL NPSLQISNFA YGEEEEEEFI HREEEMKMGF 

       250        260        270        280        290        300 
DEQEEVYDEF DDGSDSDDDE FEHSDVIEKL KTELRTARTG GLCTILEESE TPLQELKPLK 

       310        320        330        340        350        360 
IEPKPDQHKD RIAEIHKVYK NYAVKMRKLD VIDSQTMHSI SLLKLKDSSK PSRNTDKPPK 

       370        380        390        400        410        420 
SSLHQNIWPF KKHTLECDPS ERLVKEASRD FETVYVGQVC LSWEMLRWQY DKVLEFDSQV 

       430        440        450        460        470        480 
TTYQYNLVAG EFQLFQVLLQ RFVENEPFQN SSRVETYLKN RRHFQNFLQI PLVRDDRSSK 

       490        500        510        520        530        540 
KKCRYEGEFA VKTEMLREII RESMSVFWEF LCADKDEFTS MMKVSHQTQV SPQDSLDLEL 

       550        560        570        580        590        600 
LTDIRTHLQK KEKKLKEIQR SQSCIVKKLK KNESKSSIGV KDELLIAKIE LRLVSRVMYM 

       610        620        630 
SKLTTEKLHW CQEKLEKISF NGRKIHMEPY FSLLPC 

References and documentation are available.

Number of amino acids: 636

Molecular weight: 74325.13

Theoretical pI: 5.21

Amino acid composition: 
Ala (A)  12	  1.9%
Arg (R)  25	  3.9%
Asn (N)  22	  3.5%
Asp (D)  44	  6.9%
Cys (C)  11	  1.7%
Gln (Q)  31	  4.9%
Glu (E)  71	 11.2%
Gly (G)  24	  3.8%
His (H)  16	  2.5%
Ile (I)  28	  4.4%
Leu (L)  56	  8.8%
Lys (K)  63	  9.9%
Met (M)  19	  3.0%
Phe (F)  40	  6.3%
Pro (P)  17	  2.7%
Ser (S)  59	  9.3%
Thr (T)  32	  5.0%
Trp (W)   6	  0.9%
Tyr (Y)  17	  2.7%
Val (V)  43	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 115
Total number of positively charged residues (Arg + Lys): 88

Atomic composition:

Carbon      C	      3298
Hydrogen    H	      5147
Nitrogen    N	       865
Oxygen      O	      1028
Sulfur      S	        30

Formula: C3298H5147N865O1028S30
Total number of atoms: 10368

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    58955
Abs 0.1% (=1 g/l)   0.793, assuming all pairs of Cys residues form cystines


Ext. coefficient    58330
Abs 0.1% (=1 g/l)   0.785, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.85
This classifies the protein as unstable.



Aliphatic index: 73.00

Grand average of hydropathicity (GRAVY): -0.652