ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGKEKSHINV VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAELG KGSFKYAWVL 

        70         80         90        100        110        120 
DKLKAERERG ITIDIALWKF ETPKYQVTVI DAPGHRDFIK NMITGTSQAD CAILIIAGGV 

       130        140        150        160        170        180 
GEFEAGISKD GQTREHALLA FTLGVRQLIV AVNKMDSVKW DESRFQEIVK ETSNFIKKVG 

       190        200        210        220        230        240 
YNPKTVPFVP ISGWNGDNMI EATTNAPWYK GWEKETKAGV VKGKTLLEAI DAIEQPSRPT 

       250        260        270        280        290        300 
DKPLRLPLQD VYKIGGIGTV PVGRVETGVI KPGMVVTFAP AGVTTEVKSV EMHHEQLEQG 

       310        320        330        340        350        360 
VPGDNVGFNV KNVSVKEIRR GNVCGDAKND PPKGCASFNA TVIVLNHPGQ ISAGYSPVLD 

       370        380        390        400        410        420 
CHTAHIACRF DELLEKNDRR SGKKLEDHPK FLKSGDAALV KFVPSKPMCV EAFSEYPPLG 

       430        440        450 
RFAVRDMRQT VAVGVIKSVD KTEKAAKVTK AAQKAAKK 

References and documentation are available.

Number of amino acids: 458

Molecular weight: 50032.68

Theoretical pI: 9.14

Amino acid composition: 
Ala (A)  37	  8.1%
Arg (R)  18	  3.9%
Asn (N)  16	  3.5%
Asp (D)  24	  5.2%
Cys (C)   7	  1.5%
Gln (Q)  12	  2.6%
Glu (E)  31	  6.8%
Gly (G)  42	  9.2%
His (H)  11	  2.4%
Ile (I)  30	  6.6%
Leu (L)  24	  5.2%
Lys (K)  49	 10.7%
Met (M)   8	  1.7%
Phe (F)  17	  3.7%
Pro (P)  23	  5.0%
Ser (S)  21	  4.6%
Thr (T)  28	  6.1%
Trp (W)   6	  1.3%
Tyr (Y)   8	  1.7%
Val (V)  46	 10.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 67

Atomic composition:

Carbon      C	      2234
Hydrogen    H	      3585
Nitrogen    N	       617
Oxygen      O	       654
Sulfur      S	        15

Formula: C2234H3585N617O654S15
Total number of atoms: 7105

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    45295
Abs 0.1% (=1 g/l)   0.905, assuming all pairs of Cys residues form cystines


Ext. coefficient    44920
Abs 0.1% (=1 g/l)   0.898, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.42
This classifies the protein as stable.



Aliphatic index: 83.19

Grand average of hydropathicity (GRAVY): -0.300