ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG KGSFKYAWVL 

        70         80         90        100        110        120 
DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK NMITGTSQAD CAVLIVAAGV 

       130        140        150        160        170        180 
GEFEAGISKN GQTREHALLA YTLGVKQLIV GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK 

       190        200        210        220        230        240 
IGYNPDTVAF VPISGWNGDN MLEPSANMPW FKGWKVTRKD GNASGTTLLE ALDCILPPTR 

       250        260        270        280        290        300 
PTDKPLRLPL QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS 

       310        320        330        340        350        360 
EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP GQISAGYAPV 

       370        380        390        400        410        420 
LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA IVDMVPGKPM CVESFSDYPP 

       430        440        450        460 
LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK VTKSAQKAQK AK 

References and documentation are available.

Number of amino acids: 462

Molecular weight: 50140.86

Theoretical pI: 9.10

Amino acid composition: 
Ala (A)  39	  8.4%
Arg (R)  17	  3.7%
Asn (N)  16	  3.5%
Asp (D)  26	  5.6%
Cys (C)   6	  1.3%
Gln (Q)  10	  2.2%
Glu (E)  27	  5.8%
Gly (G)  44	  9.5%
His (H)  10	  2.2%
Ile (I)  30	  6.5%
Leu (L)  26	  5.6%
Lys (K)  47	 10.2%
Met (M)  12	  2.6%
Phe (F)  14	  3.0%
Pro (P)  25	  5.4%
Ser (S)  23	  5.0%
Thr (T)  29	  6.3%
Trp (W)   5	  1.1%
Tyr (Y)  12	  2.6%
Val (V)  44	  9.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 64

Atomic composition:

Carbon      C	      2235
Hydrogen    H	      3589
Nitrogen    N	       611
Oxygen      O	       659
Sulfur      S	        18

Formula: C2235H3589N611O659S18
Total number of atoms: 7112

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    45755
Abs 0.1% (=1 g/l)   0.913, assuming all pairs of Cys residues form cystines


Ext. coefficient    45380
Abs 0.1% (=1 g/l)   0.905, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 27.03
This classifies the protein as stable.



Aliphatic index: 83.33

Grand average of hydropathicity (GRAVY): -0.257