ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGKEKTHVNV VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAELG KGSFKYAWVL 

        70         80         90        100        110        120 
DKLKAERERG ITIDIALWKF ETPKYHVTVI DAPGHRDFIK NMITGTSQAD CGILIIAAGT 

       130        140        150        160        170        180 
GEFEAGISKD GQTREHALLA FTLGVRQLIV AINKMDSTKW SEQRFNEIIK EVSGFIKKIG 

       190        200        210        220        230        240 
FNPKSVPFVP ISGWHGDNML EESTNMPWYK GWNKETKAGA KSGKTLLDAI DAIDPPQRPS 

       250        260        270        280        290        300 
DKPLRLPLQD VYKIGGIGTV PVGRVETGVI KAGMVVTFAP ANVTTEVKSV EMHHEQLVEG 

       310        320        330        340        350        360 
LPGDNVGFNV KNVSVKDIRR GNVCSDSKND PAKEAGSFTA QVIVLNHPGQ IGAGYAPVLD 

       370        380        390        400        410        420 
CHTAHIACKF AELLEKIDRR SGKKLEDAPK FVKSGDSAIV KMIPSKPMCV EAYTDYPPLG 

       430        440        450 
RFAVRDMRQT VAVGVIKAVE KVDKAGKVTK AAAKAGEK 

References and documentation are available.

Number of amino acids: 458

Molecular weight: 49828.51

Theoretical pI: 9.12

Amino acid composition: 
Ala (A)  39	  8.5%
Arg (R)  17	  3.7%
Asn (N)  15	  3.3%
Asp (D)  26	  5.7%
Cys (C)   6	  1.3%
Gln (Q)  10	  2.2%
Glu (E)  29	  6.3%
Gly (G)  44	  9.6%
His (H)  12	  2.6%
Ile (I)  34	  7.4%
Leu (L)  24	  5.2%
Lys (K)  49	 10.7%
Met (M)  10	  2.2%
Phe (F)  16	  3.5%
Pro (P)  22	  4.8%
Ser (S)  22	  4.8%
Thr (T)  27	  5.9%
Trp (W)   6	  1.3%
Tyr (Y)   8	  1.7%
Val (V)  42	  9.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      2224
Hydrogen    H	      3573
Nitrogen    N	       613
Oxygen      O	       651
Sulfur      S	        16

Formula: C2224H3573N613O651S16
Total number of atoms: 7077

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    45295
Abs 0.1% (=1 g/l)   0.909, assuming all pairs of Cys residues form cystines


Ext. coefficient    44920
Abs 0.1% (=1 g/l)   0.901, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.80
This classifies the protein as stable.



Aliphatic index: 84.50

Grand average of hydropathicity (GRAVY): -0.265