ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGKEKVHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAQEMG KGSFKYAWVL 

        70         80         90        100        110        120 
DKLKAERERG ITIDIALWKF ETAKYYITII DAPGHRDFIK NMITGTSQAD CAVLVVACNK 

       130        140        150        160        170        180 
MDSTEPPFSE ARFTEITNEV SGFIKKIGYN PKAVPFVPIS GFNGDNMLEV SSNMPWFKGW 

       190        200        210        220        230        240 
AVERKEGNAS GKTLLEALDS IIPPQRPTDR PLRLPLQDVY KIGGIGTVPV GRVETGIIKP 

       250        260        270        280        290        300 
GMVVTFAPQN VTTEVKSVEM HHESLPEAVP GDNVGFNVKN VSVKDIRRGS VCSDSKQDPA 

       310        320        330        340        350        360 
KEARTFHAQV IIMNHPGQIS NGYTPVLDCH TAHIACKFNE LKEKVDRRTG KKVEDFPKFL 

       370        380        390        400        410        420 
KSGDAGIVEL IPTKPLCVES FTDYAPLGRF AVRDMRQTVA VGVIKSVEKS DGSSGKVTKS 


AQKAAPKKK 

References and documentation are available.

Number of amino acids: 429

Molecular weight: 47145.34

Theoretical pI: 9.12

Amino acid composition: 
Ala (A)  28	  6.5%
Arg (R)  18	  4.2%
Asn (N)  16	  3.7%
Asp (D)  23	  5.4%
Cys (C)   7	  1.6%
Gln (Q)  10	  2.3%
Glu (E)  28	  6.5%
Gly (G)  36	  8.4%
His (H)  10	  2.3%
Ile (I)  32	  7.5%
Leu (L)  19	  4.4%
Lys (K)  44	 10.3%
Met (M)  10	  2.3%
Phe (F)  18	  4.2%
Pro (P)  25	  5.8%
Ser (S)  26	  6.1%
Thr (T)  27	  6.3%
Trp (W)   4	  0.9%
Tyr (Y)   8	  1.9%
Val (V)  40	  9.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 62

Atomic composition:

Carbon      C	      2100
Hydrogen    H	      3365
Nitrogen    N	       577
Oxygen      O	       619
Sulfur      S	        17

Formula: C2100H3365N577O619S17
Total number of atoms: 6678

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    34295
Abs 0.1% (=1 g/l)   0.727, assuming all pairs of Cys residues form cystines


Ext. coefficient    33920
Abs 0.1% (=1 g/l)   0.719, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.82
This classifies the protein as stable.



Aliphatic index: 79.93

Grand average of hydropathicity (GRAVY): -0.328