ProtParam
User-provided sequence:
10 20 30 40 50 60
MGKEKVHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAQEMG KGSFKYAWVL
70
DKLKVTKSAQ KAAPKKK
References and
documentation are available.
Number of amino acids: 77
Molecular weight: 8546.06
Theoretical pI: 9.82
Total number of negatively charged residues (Asp + Glu): 8
Total number of positively charged residues (Arg + Lys): 17
Atomic composition:
Carbon C 383
Hydrogen H 632
Nitrogen N 106
Oxygen O 108
Sulfur S 3
Formula: C
383H
632N
106O
108S
3
Total number of atoms: 1232
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 8480
Abs 0.1% (=1 g/l) 0.992, assuming all pairs of Cys residues form cystines
Ext. coefficient 8480
Abs 0.1% (=1 g/l) 0.992, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 22.96
This classifies the protein as stable.
Aliphatic index: 74.68
Grand average of hydropathicity (GRAVY): -0.591