ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGKENAVSRP FTRSLASALR ASEVTSTTQN QQRVNTKRPA LEDTRATGPN KRKKRAVLGE 

        70         80         90        100        110        120 
ITNVNSNTAI LEAKNSKQIK KGRGHGLAST SQLATSVTSE VTDLQSRTDA KVEVASNTAG 

       130        140        150        160        170        180 
NLSVSKGTDN TADNCIEIWN SRLPPRPLGR SASTAEKSAV IGSSTVPDIP KFVDIDSDDK 

       190        200        210        220        230        240 
DPLLCCLYAP EIHYNLRVSE LKRRPLPDFM ERIQKDVTQS MRGILVDWLV EVSEEYTLAS 

       250        260        270        280        290        300 
DTLYLTVYLI DWFLHGNYVQ RQQLQLLGIT CMLIASKYEE ISAPRIEEFC FITDNTYTRD 

       310        320        330        340        350        360 
QVLEMENQVL KHFSFQIYTP TPKTFLRRFL RAAQASRLSP SLEVEFLASY LTELTLIDYH 

       370        380        390        400        410        420 
FLKFLPSVVA ASAVFLAKWT MDQSNHPWNP TLEHYTTYKA SDLKASVHAL QDLQLNTKGC 

       430        440        450 
PLSAIRMKYR QEKYKSVAVL TSPKLLDTLF 

References and documentation are available.

Number of amino acids: 450

Molecular weight: 50641.75

Theoretical pI: 8.82

Amino acid composition: 
Ala (A)  35	  7.8%
Arg (R)  27	  6.0%
Asn (N)  20	  4.4%
Asp (D)  23	  5.1%
Cys (C)   6	  1.3%
Gln (Q)  20	  4.4%
Glu (E)  26	  5.8%
Gly (G)  14	  3.1%
His (H)   8	  1.8%
Ile (I)  20	  4.4%
Leu (L)  51	 11.3%
Lys (K)  28	  6.2%
Met (M)   7	  1.6%
Phe (F)  15	  3.3%
Pro (P)  21	  4.7%
Ser (S)  41	  9.1%
Thr (T)  39	  8.7%
Trp (W)   5	  1.1%
Tyr (Y)  15	  3.3%
Val (V)  29	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2246
Hydrogen    H	      3592
Nitrogen    N	       620
Oxygen      O	       684
Sulfur      S	        13

Formula: C2246H3592N620O684S13
Total number of atoms: 7155

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    50225
Abs 0.1% (=1 g/l)   0.992, assuming all pairs of Cys residues form cystines


Ext. coefficient    49850
Abs 0.1% (=1 g/l)   0.984, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.02
This classifies the protein as unstable.



Aliphatic index: 88.00

Grand average of hydropathicity (GRAVY): -0.338