ProtParam
User-provided sequence:
10 20 30 40 50 60
MGKFSKIKKV QEEESAHQKM EWEAAGAKDS SSDDSSDESD NDDQPKQATE ETRKRAELWT
70 80 90 100 110 120
NRERVLVLCS RGADVRTRYL MKDIKDLLPH AKGDSKLDQQ KSLNVLNEIA EMKNCTKVMY
130 140 150 160 170 180
FESRKRKDTY LWMSNVEKGP SIKFLVHNVH TMKELKMSGN CLRASRPVLS FDDAFDKKPQ
190 200 210 220 230 240
LKLIKAVLMQ TLGTPHHHPR SQPFVDHVFN FSVGEGDKIW FRNFQIVDES LQLQEVGPRF
250 260 270 280 290 300
VLEMVRLFAG SFEGAVLYDN PNYVSPNVIR REHRKGQHSY IEKQLAVKAS NIKQAKVTEI
310 320 330 340 350
LAEKTVDLVG KEFDTQNNAA ADSEAAAQIT AQIEKRRVRK KKSQASKYTG SD
References and
documentation are available.
Number of amino acids: 352
Molecular weight: 40233.70
Theoretical pI: 9.12
Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 59
Atomic composition:
Carbon C 1763
Hydrogen H 2824
Nitrogen N 512
Oxygen O 539
Sulfur S 13
Formula: C
1763H
2824N
512O
539S
13
Total number of atoms: 5651
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 32555
Abs 0.1% (=1 g/l) 0.809, assuming all pairs of Cys residues form cystines
Ext. coefficient 32430
Abs 0.1% (=1 g/l) 0.806, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.23
This classifies the protein as unstable.
Aliphatic index: 72.02
Grand average of hydropathicity (GRAVY): -0.753