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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGKGTPSFGK RHNKSHTLCN RCGRRSFHVQ KKTCSSCGYP AAKTRSYNWG AKAKRRHTTG

70 80
TGRMRYLKHV SRRFKNGFQT GSASKASA


References and documentation are available.

Number of amino acids: 88 Molecular weight: 9850.27 Theoretical pI: 11.64
Amino acid composition: 
Ala (A) 7 8.0% Arg (R) 11 12.5% Asn (N) 4 4.5% Asp (D) 0 0.0% Cys (C) 4 4.5% Gln (Q) 2 2.3% Glu (E) 0 0.0% Gly (G) 10 11.4% His (H) 5 5.7% Ile (I) 0 0.0% Leu (L) 2 2.3% Lys (K) 11 12.5% Met (M) 2 2.3% Phe (F) 4 4.5% Pro (P) 2 2.3% Ser (S) 10 11.4% Thr (T) 8 9.1% Trp (W) 1 1.1% Tyr (Y) 3 3.4% Val (V) 2 2.3% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 0 Total number of positively charged residues (Arg + Lys): 22 Atomic composition: Carbon C 419 Hydrogen H 677 Nitrogen N 149 Oxygen O 116 Sulfur S 6 Formula: C419H677N149O116S6 Total number of atoms: 1367 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 10220 Abs 0.1% (=1 g/l) 1.038, assuming all pairs of Cys residues form cystines Ext. coefficient 9970 Abs 0.1% (=1 g/l) 1.012, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 50.62 This classifies the protein as unstable. Aliphatic index: 23.41 Grand average of hydropathicity (GRAVY): -1.152