ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGKHLFRSSP PPEKPTAHLS LAESAVQECM SNINTVISKW TSPAASSSDD FLFSTNSRRE 

        70         80         90        100        110        120 
AEEFVTAVRH LQSTMHRLVS VNPSSEKLIY AQNLMQSAMK LLESEFHRVL KANREYLDPE 

       130        140        150        160        170        180 
CVSVRSYRSS RFSTSTTTSV SDSEDESSYE ENADEEHRFS GGDSDAMDDL KMIADCMIST 

       190        200        210        220        230        240 
GYAKECVRVY KTVRKSIVDE TLHNLQMERF NLHQVQKMDW EILESKIKTW LKAVKLAVRK 

       250        260        270        280        290        300 
LFFGERILAD HVFSSSGLIV ESSFTEITQE GALILFTFPE YASKIKKLTP EKMFRFLDMY 

       310        320        330        340        350        360 
EALANLYVEI ESIFYFESAA AVRSQVINSL ARLGDATRLM MTDFESAIQK ETSKTPIIGG 

       370        380        390        400        410        420 
GVHPLTRYVM NYLSFLADYS DSIAAIFENW KLSVPTPLPD SLYISGGDEA NPEDLYSSPV 

       430        440        450        460        470        480 
SVRIAWVILL TLCKIDGKAQ PYKDVALSYL FLANNLQYVV VKVRSSTLKV LLGDDWVFRH 

       490        500        510        520        530        540 
EEKVKLYADK FEKLAWGKVL DLLPEIPTDE ISPEEAKVLV ARFNDEFETS YRKQTSWVIP 

       550        560        570        580        590        600 
DPKLRDQIKI TLSQKLMLVC TEFYRMNRFA YGMVGDNEAI SRYTPEDIGN YLSDLYFGSR 

       610        620        630 
GSGSVSTAGG SGSGSGSGTG SSSTGKARGG RSSH 

References and documentation are available.

Number of amino acids: 634

Molecular weight: 71225.81

Theoretical pI: 5.64

Amino acid composition: 
Ala (A)  43	  6.8%
Arg (R)  33	  5.2%
Asn (N)  20	  3.2%
Asp (D)  35	  5.5%
Cys (C)   6	  0.9%
Gln (Q)  15	  2.4%
Glu (E)  49	  7.7%
Gly (G)  32	  5.0%
His (H)  12	  1.9%
Ile (I)  32	  5.0%
Leu (L)  60	  9.5%
Lys (K)  39	  6.2%
Met (M)  18	  2.8%
Phe (F)  29	  4.6%
Pro (P)  23	  3.6%
Ser (S)  77	 12.1%
Thr (T)  35	  5.5%
Trp (W)   8	  1.3%
Tyr (Y)  25	  3.9%
Val (V)  43	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 84
Total number of positively charged residues (Arg + Lys): 72

Atomic composition:

Carbon      C	      3172
Hydrogen    H	      4967
Nitrogen    N	       839
Oxygen      O	       975
Sulfur      S	        24

Formula: C3172H4967N839O975S24
Total number of atoms: 9977

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    81625
Abs 0.1% (=1 g/l)   1.146, assuming all pairs of Cys residues form cystines


Ext. coefficient    81250
Abs 0.1% (=1 g/l)   1.141, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.01
This classifies the protein as unstable.



Aliphatic index: 83.04

Grand average of hydropathicity (GRAVY): -0.269