ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGKIAEFLGN PGARPDVHHR APTVDCKQYE EFGDSNDYKN DDVVRVVSHS DESTDDELCN 

        70         80         90        100        110        120 
VNLTETGAIF TSKGFTGLSK GFTDKTLDFL VRVAGSQAVF FIVWIILIIW VVIGIVYNAP 

       130        140        150        160        170        180 
FNWQVVMQDG QSIQSYVWDT LLMRQQLMST HEQILICGRL KSRLASFKNY LTRSTPEEEK 

       190        200        210        220        230        240 
ADCTVEANEV SSVENHIDPS AINGELPVEN WYDRLSNVAS RYMGSIAAMV IFWIGIFVWI 

       250        260        270        280        290        300 
GCGAIPKDAG NTPPYTGETT GSNPRLKKFS DAWQMYINTA VAVSLLICTT FLQNIRARHD 

       310        320        330        340        350        360 
YFTGRFLVDI FDMDEKIDYR IRKHFNDFET PHPVVTIESK KRSTGRKMID WYADIIGTGI 

       370        380        390        400        410        420 
GVLIGVAVFA TWIGIGSPMK WDDNWWLIIG TYTGLIGFLD GFVLREVYFR IVQHEEKNYS 

       430        440        450        460        470        480 
DVAKEDLELF QELGIECPEE FSGKAPEINT IGYRTSQYIN RICSTPWSVL VSVIIIIGLI 

       490        500        510        520        530        540 
CIASGLRWST TGQLIANTPT MIIEEFFLLV LLQAHNWADR QRRVEVTALY ARRRILLSYV 

       550 
EKRFPEVMML EK 

References and documentation are available.

Number of amino acids: 552

Molecular weight: 62791.99

Theoretical pI: 5.32

Amino acid composition: 
Ala (A)  31	  5.6%
Arg (R)  30	  5.4%
Asn (N)  24	  4.3%
Asp (D)  32	  5.8%
Cys (C)   9	  1.6%
Gln (Q)  17	  3.1%
Glu (E)  35	  6.3%
Gly (G)  38	  6.9%
His (H)  10	  1.8%
Ile (I)  53	  9.6%
Leu (L)  41	  7.4%
Lys (K)  23	  4.2%
Met (M)  13	  2.4%
Phe (F)  28	  5.1%
Pro (P)  19	  3.4%
Ser (S)  34	  6.2%
Thr (T)  36	  6.5%
Trp (W)  16	  2.9%
Tyr (Y)  19	  3.4%
Val (V)  44	  8.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2847
Hydrogen    H	      4392
Nitrogen    N	       742
Oxygen      O	       817
Sulfur      S	        22

Formula: C2847H4392N742O817S22
Total number of atoms: 8820

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   116810
Abs 0.1% (=1 g/l)   1.860, assuming all pairs of Cys residues form cystines


Ext. coefficient   116310
Abs 0.1% (=1 g/l)   1.852, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.42
This classifies the protein as stable.



Aliphatic index: 95.14

Grand average of hydropathicity (GRAVY): -0.020