ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGKKVKIGKQ RRDKYYKLAK EAGYRSRAAF KLVQLNKRFE FLEKSRATVD LCAAPGGWMQ 

        70         80         90        100        110        120 
VASQFMPVSS LIVGVDLAPI KPIKNCIALQ GDITTNETRA AIKKELKTWS ADCVLHDGAP 

       130        140        150        160        170        180 
NVGLNWVHDA FQQNCLTLSA LKLATQILRK GGTFVTKVFR SNDYSCLIRV FEKLFKRVHV 

       190        200        210        220        230        240 
WKPAASRLES AEIFVVCEVY QKPDKVGAEY LDPKKVFANP DGSEGTKPNP QNLLIGKQKK 

       250        260        270        280        290        300 
AKAEGYDTDS LAIHSTINAT DFIKSSGYLD ILGTANVITL DDEKWKNHEK TTEEVIEYMN 

       310        320        330        340        350        360 
DVKVLGPREL RVLLRWRKSM LETLEEQRKA VEGEAKEVEI EENLTEEQLE DRAMAEIDEL 

       370        380        390        400        410        420 
IAKASEDEKA ALKKKKKKML KAKARVLKRR ELKMIIDGDE GPQAEDQEVF QLKKIRKAKE 

       430        440        450        460        470        480 
LAEITKDTQA PDFDMMEDGD SDEEEGLGEG EWETHGEEGE NSDEDENELI HTEKSGMSKM 

       490        500        510        520        530        540 
EKRNARTESW FEKEEISGLV SDEDDDDEMN AIEKHIGKNR SKKTENYENT VSFDDSKGKK 

       550        560        570        580        590        600 
KGKKGGKGGE DDGFNTNDAD EQASESESSD AEMDEVAKEK LSRFDAQIDL DDDDEEERYE 

       610        620        630        640        650        660 
DEGRRAAKRK CDKKIIGEDL KPVAKKRKLT PEQLAIGEQM IYSAKAARNL EDEAWNRYAN 

       670        680        690        700        710 
NDEDLPDWFA DDEKKHYFKQ TPVTKEQVAL YRERMREFNA RPSKKVAEAK ARKQKK 

References and documentation are available.

Number of amino acids: 716

Molecular weight: 81525.82

Theoretical pI: 5.42

Amino acid composition: 
Ala (A)  63	  8.8%
Arg (R)  38	  5.3%
Asn (N)  29	  4.1%
Asp (D)  60	  8.4%
Cys (C)   7	  1.0%
Gln (Q)  24	  3.4%
Glu (E)  87	 12.2%
Gly (G)  41	  5.7%
His (H)   9	  1.3%
Ile (I)  33	  4.6%
Leu (L)  52	  7.3%
Lys (K)  91	 12.7%
Met (M)  16	  2.2%
Phe (F)  21	  2.9%
Pro (P)  19	  2.7%
Ser (S)  35	  4.9%
Thr (T)  30	  4.2%
Trp (W)  10	  1.4%
Tyr (Y)  15	  2.1%
Val (V)  36	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 147
Total number of positively charged residues (Arg + Lys): 129

Atomic composition:

Carbon      C	      3555
Hydrogen    H	      5706
Nitrogen    N	      1002
Oxygen      O	      1144
Sulfur      S	        23

Formula: C3555H5706N1002O1144S23
Total number of atoms: 11430

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    77725
Abs 0.1% (=1 g/l)   0.953, assuming all pairs of Cys residues form cystines


Ext. coefficient    77350
Abs 0.1% (=1 g/l)   0.949, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.31
This classifies the protein as stable.



Aliphatic index: 69.68

Grand average of hydropathicity (GRAVY): -0.924