ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGKLAVAAIT SLWVIPMSII VNHIVPEPYM DEIFHVPQAQ QYCNGNFRSW DPMITTPPGL 

        70         80         90        100        110        120 
YYLSLAHVAS LFPGMLLMEN TSQSFSEACS TSVLRSTNAV SAVLCGVLVY EIIRFLGPNL 

       130        140        150        160        170        180 
SDRKATFMAL VMSLYPLHWF FTFLYYTDVA SLTAVLAMYL TCLKRRYVLS ALFGTLAVFI 

       190        200        210        220        230        240 
RQTNVVWMLF VACSGILDFT LDSSKQKGKQ EVNQELHQSS NKKGATLRSN LRKRKSDISS 

       250        260        270        280        290        300 
DTSDPFNHGQ TVPSTEDTSD LVYDIYTVIS TSWNLKWRIL IKFSPFIFVV VAFGIFILWN 

       310        320        330        340        350        360 
GGIVLGAKEA HVVSLHFAQI MYFSLVSALF TAPLHFSVNQ LRHQFHQLHR NWSLSLILTL 

       370        380        390        400        410        420 
VALVAGFVSV HFFSLAHPYL LADNRHYPFY LWRKIINAHW LMKYILVPVY VYSWFSILTL 

       430        440        450        460        470        480 
LAKTRRQTWI LVYFLATCGV LVPTPLIEFR YYTIPFYLFM LHSCVRSSSF ATWLLIGTIF 

       490        500 
VSINVFTMAM FLFRPFKWSH EDGVQRFIW 

References and documentation are available.

Number of amino acids: 509

Molecular weight: 58438.60

Theoretical pI: 9.34

Amino acid composition: 
Ala (A)  33	  6.5%
Arg (R)  21	  4.1%
Asn (N)  18	  3.5%
Asp (D)  14	  2.8%
Cys (C)   7	  1.4%
Gln (Q)  16	  3.1%
Glu (E)  11	  2.2%
Gly (G)  19	  3.7%
His (H)  18	  3.5%
Ile (I)  33	  6.5%
Leu (L)  64	 12.6%
Lys (K)  17	  3.3%
Met (M)  15	  2.9%
Phe (F)  40	  7.9%
Pro (P)  21	  4.1%
Ser (S)  47	  9.2%
Thr (T)  32	  6.3%
Trp (W)  15	  2.9%
Tyr (Y)  23	  4.5%
Val (V)  45	  8.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      2745
Hydrogen    H	      4154
Nitrogen    N	       674
Oxygen      O	       696
Sulfur      S	        22

Formula: C2745H4154N674O696S22
Total number of atoms: 8291

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   117145
Abs 0.1% (=1 g/l)   2.005, assuming all pairs of Cys residues form cystines


Ext. coefficient   116770
Abs 0.1% (=1 g/l)   1.998, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.16
This classifies the protein as unstable.



Aliphatic index: 106.44

Grand average of hydropathicity (GRAVY): 0.449