ProtParam
User-provided sequence:
10 20 30 40 50 60
MGKLLQLALH PVEMKAALKL KFCRTPLFSI YDQSTSPYLL HCFELLNLTS RSFAAVIREL
70 80 90 100 110 120
HPELRNCVTL FYLILRALDT IEDDMSIEHD LKIDLLRHFH EKLLLTKWSF DGNAPDVKDR
130 140 150 160 170 180
AVLTDFESIL IEFHKLKPEY QEVIKEITEK MGNGMADYIL DENYNLNGLQ TVHDYDVYCH
190 200 210 220 230 240
YVAGLVGDGL TRLIVIAKFA NESLYSNEQL YESMGLFLQK TNIIRDYNED LVDGRSFWPK
250 260 270 280 290 300
EIWSQYAPQL KDFMKPENEQ LGLDCINHLV LNALSHVIDV LTYLAGIHEQ STFQFCAIPQ
310 320 330 340 350 360
VMAIATLALV FNNREVLHGN VKIRKGTTCY LILKSRTLRG CVEIFDYYLR DIKSKLAVQD
370 380 390 400 410 420
PNFLKLNIQI SKIEQFMEEM YQDKLPPNVK PNETPIFLKV KERSRYDDEL VPTQQEEEYK
430 440
FNMVLSIILS VLLGFYYIYT LHRA
References and
documentation are available.
Number of amino acids: 444
Molecular weight: 51719.90
Theoretical pI: 5.58
Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 47
Atomic composition:
Carbon C 2358
Hydrogen H 3680
Nitrogen N 598
Oxygen O 669
Sulfur S 19
Formula: C
2358H
3680N
598O
669S
19
Total number of atoms: 7324
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 51270
Abs 0.1% (=1 g/l) 0.991, assuming all pairs of Cys residues form cystines
Ext. coefficient 50770
Abs 0.1% (=1 g/l) 0.982, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.07
This classifies the protein as unstable.
Aliphatic index: 106.04
Grand average of hydropathicity (GRAVY): -0.107