ProtParam
User-provided sequence:
10 20 30 40 50 60
MGKLQVIEKK FEFGFRVFYN LFRLFPLIYS NTWLTLVLTA VTIAASVGNE IVNYKTGVIP
70 80 90 100 110 120
GKFYVALIEK DEPKFWHIFW IASLCYVGLC LLMALMNFFS WCLYIQQRKN LVQCLHKLYY
130 140 150 160 170 180
KNNFYYQLNG VDDHGIDNPD QRITQDADKL TKLLGTSIIP TVLLSPFIIS YYGVRVWETA
190 200 210 220 230 240
GGWGFGMIVA YFLIGVIINR ILIGPITPWA ARVEKAEGDF RYKHVSVRVN AEESAFYKSA
250 260 270 280 290 300
EFEHVFSDLS FDVLFKRLLN FMCWRFPSQF FQSFFDYYGG CMSYILQLFP LFVFHMYDNL
310 320 330 340 350 360
TPAELSGQIS NNAFYFIYLI NCFTRLTDLA MNIAELGGYI MRVSEFVDTG KLHQSGIKNE
370 380 390 400 410 420
AFRRNSELPE KASGDDNLIE TEHLSFGPPT NSNIRIIENL TFHLPRNKTL LITGDSGIGK
430 440 450 460 470 480
TSLMRVIADM WPHQGILKKH FKKTDAYFLP QKPYFPVGRL SLKQQLVFPA EENEEKSGND
490 500 510 520 530 540
EEIVRILHEL KLGHLLPIVG NIHDSVDFEW QETLSPGEQQ RLCFARMILA EPTVAFLDES
550 560 570 580 590
TCNVDEGMEN AMYEMLRKHS ITYISVGHRK SLRSHHDFEL RIPSSSSFLF HEIHNQKF
References and
documentation are available.
Number of amino acids: 598
Molecular weight: 69172.88
Theoretical pI: 6.48
Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 58
Atomic composition:
Carbon C 3192
Hydrogen H 4839
Nitrogen N 809
Oxygen O 866
Sulfur S 24
Formula: C
3192H
4839N
809O
866S
24
Total number of atoms: 9730
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 94240
Abs 0.1% (=1 g/l) 1.362, assuming all pairs of Cys residues form cystines
Ext. coefficient 93740
Abs 0.1% (=1 g/l) 1.355, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.97
This classifies the protein as stable.
Aliphatic index: 93.56
Grand average of hydropathicity (GRAVY): -0.028