ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGKNKKKTGK KAKSHPHVED VDETVNKPEE IINSVNVTVP PKMSTDPEAD GIVASPDDEG 

        70         80         90        100        110        120 
KDLSEGVDKQ KVNDGLTVDT INPLEDKKAG DEMKELREEI ERLKLELSHK KDQETPNEDF 

       130        140        150        160        170        180 
KNELANVIKE RDEFKTQYDT LLSKISSMKS IFNKMKEAQK QLEEVQEQLT EYESQNLKLK 

       190        200        210        220        230        240 
KKLEATKTEN SELQSTIVTL NTELENLEKE QESTEEVFLE YESRIEALED EKHDIIEKHS 

       250        260        270        280        290        300 
KELNTYRKEK DQLNLQVQEL MIILENNKQD ISDLRTERDE LRQALESHEK EKAVLKNSLN 

       310        320        330        340        350        360 
DLELKIEEVD NKREEEARER DQEVKSLRSQ LDTEIETHNN DTEALESMKK QLEAMKEDAS 

       370        380        390        400        410        420 
MKEKYEEESK QHILQIGKLR HEAIILNEHL TKALAMLKKS SDSESVDKEL ISNLLISFVS 

       430        440        450        460        470        480 
IPRADPRKFE VLELLSNFLN WDEDKKQQAG LISNNESKNS SAVSRTESFV SLWTNYLEKE 


SEKD 

References and documentation are available.

Number of amino acids: 484

Molecular weight: 56068.56

Theoretical pI: 4.85

Amino acid composition: 
Ala (A)  20	  4.1%
Arg (R)  16	  3.3%
Asn (N)  31	  6.4%
Asp (D)  34	  7.0%
Cys (C)   0	  0.0%
Gln (Q)  22	  4.5%
Glu (E)  80	 16.5%
Gly (G)   9	  1.9%
His (H)  10	  2.1%
Ile (I)  25	  5.2%
Leu (L)  53	 11.0%
Lys (K)  61	 12.6%
Met (M)  10	  2.1%
Phe (F)   8	  1.7%
Pro (P)  10	  2.1%
Ser (S)  40	  8.3%
Thr (T)  24	  5.0%
Trp (W)   2	  0.4%
Tyr (Y)   6	  1.2%
Val (V)  23	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 114
Total number of positively charged residues (Arg + Lys): 77

Atomic composition:

Carbon      C	      2417
Hydrogen    H	      3954
Nitrogen    N	       668
Oxygen      O	       836
Sulfur      S	        10

Formula: C2417H3954N668O836S10
Total number of atoms: 7885

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    19940
Abs 0.1% (=1 g/l)   0.356

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.46
This classifies the protein as unstable.



Aliphatic index: 80.76

Grand average of hydropathicity (GRAVY): -1.067