ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGKRKKSTRK PTKRLVQKLD TKFNCLFCNH EKSVSCTLDK KNSIGTLSCK ICGQSFQTRI 

        70         80         90        100        110        120 
NSLSQPVDVY SDWFDAVEEV NSGRGSDTDD GDEGSDSDYE SDSEQDAKTQ NDGEIDSDEE 

       130        140 
EVDSDEERIG QVKRGRGALV DSDDE 

References and documentation are available.

Number of amino acids: 145

Molecular weight: 16157.37

Theoretical pI: 4.48

Amino acid composition: 
Ala (A)   3	  2.1%
Arg (R)   8	  5.5%
Asn (N)   6	  4.1%
Asp (D)  21	 14.5%
Cys (C)   5	  3.4%
Gln (Q)   7	  4.8%
Glu (E)  13	  9.0%
Gly (G)  11	  7.6%
His (H)   1	  0.7%
Ile (I)   5	  3.4%
Leu (L)   7	  4.8%
Lys (K)  13	  9.0%
Met (M)   1	  0.7%
Phe (F)   4	  2.8%
Pro (P)   2	  1.4%
Ser (S)  18	 12.4%
Thr (T)   8	  5.5%
Trp (W)   1	  0.7%
Tyr (Y)   2	  1.4%
Val (V)   9	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 21

Atomic composition:

Carbon      C	       669
Hydrogen    H	      1068
Nitrogen    N	       198
Oxygen      O	       255
Sulfur      S	         6

Formula: C669H1068N198O255S6
Total number of atoms: 2196

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     8730
Abs 0.1% (=1 g/l)   0.540, assuming all pairs of Cys residues form cystines


Ext. coefficient     8480
Abs 0.1% (=1 g/l)   0.525, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.92
This classifies the protein as unstable.



Aliphatic index: 52.34

Grand average of hydropathicity (GRAVY): -1.156