ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGKTQKKNSK GRLDRYYYLA KEKGYRARSS FKIIQINEKY GHFLEKSKVV IDLCAAPGSW 

        70         80         90        100        110        120 
CQVASKLCPV NSLIIGVDIV PMKPMPNVIT FQSDITTEDC RSKLRGYMKT WKADTVLHDG 

       130        140        150        160        170        180 
APNVGLGWVQ DAFTQSQLTL QALKLAVENL VVNGTFVTKI FRSKDYNKLI WVFQQLFEKV 

       190        200        210        220        230        240 
EATKPPASRN VSAEIFVVCK GFKAPKRLDP RLLDPKEVFE ELPDGQQNME SKIYNPEKKV 

       250        260        270        280        290        300 
RKRQGYEEGD NLLYHETSIL DFVRTEDPIS MLGEMNKFTI DENDHEWKIL KKLKQTTDEF 

       310        320        330        340        350        360 
RSCIEDLKVL GKKDFKMILR WRKIAREILG IEVKDDAKTE IEVVPLTEEE QIEKDLQGLQ 

       370        380        390        400        410        420 
EKQRLNVKRE RRRKNEMKQK ELQRMQMNMI TPTDIGIEAA SLGKESLFNL KTAEKTGILN 

       430        440        450        460        470        480 
DLAKGKKRMI FTDDELAKDN DIYIDENIMI KDKDSAADAD DLESELNAMY SDYKTRRSER 

       490        500        510        520        530        540 
DAKFRAKQAR GGDNEEEWTG FNEGSLEKKE EEGKDYIEDN DDEGVEGDSD DDEAITNLIS 

       550        560        570        580        590        600 
KLKGQEGDHK LSSKARMIFN DPIFNNVEPD LPVNTVNDGI MSSESVGDIS KLNKKRKHEE 

       610        620        630        640        650        660 
MHQKQDEADS SDESSSDDSD FEIVANDNAS EEFDSDYDSE EEKNQTKKEK HSRDIDIATV 

       670        680        690        700        710        720 
EAMTLAHQLA LGQKNKHDLV DEGFNRYTFR DTENLPDWFL EDEKEHSKIN KPITKEAAMA 

       730        740        750        760        770        780 
IKEKIKAMNA RPIKKVAEAK ARKRMRAVAR LEKIKKKAGL INDDSDKTEK DKAEEISRLM 

       790        800        810        820        830        840 
RKVTKKPKTK PKVTLVVASG RNKGLAGRPK GVKGKYKMVD GVMKNEQRAL RRIAKKHHKK 


K 

References and documentation are available.

Number of amino acids: 841

Molecular weight: 96484.76

Theoretical pI: 8.08

Amino acid composition: 
Ala (A)  53	  6.3%
Arg (R)  46	  5.5%
Asn (N)  43	  5.1%
Asp (D)  73	  8.7%
Cys (C)   6	  0.7%
Gln (Q)  29	  3.4%
Glu (E)  82	  9.8%
Gly (G)  43	  5.1%
His (H)  13	  1.5%
Ile (I)  52	  6.2%
Leu (L)  61	  7.3%
Lys (K) 112	 13.3%
Met (M)  25	  3.0%
Phe (F)  26	  3.1%
Pro (P)  25	  3.0%
Ser (S)  46	  5.5%
Thr (T)  37	  4.4%
Trp (W)   8	  1.0%
Tyr (Y)  17	  2.0%
Val (V)  44	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 155
Total number of positively charged residues (Arg + Lys): 158

Atomic composition:

Carbon      C	      4217
Hydrogen    H	      6837
Nitrogen    N	      1197
Oxygen      O	      1324
Sulfur      S	        31

Formula: C4217H6837N1197O1324S31
Total number of atoms: 13606

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    69705
Abs 0.1% (=1 g/l)   0.722, assuming all pairs of Cys residues form cystines


Ext. coefficient    69330
Abs 0.1% (=1 g/l)   0.719, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.95
This classifies the protein as unstable.



Aliphatic index: 73.88

Grand average of hydropathicity (GRAVY): -0.889