ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGKVGAGDGS SAGLSALLAG AGLLMLLAPG VCSSLSCCPP QHPSSTPRRT LTPRGFPHPG 

        70         80         90        100        110        120 
PLGRAPATPP PLFMRPLFAV APGDRALFLE RAGGSRVSVA TAARSGRRRR SGTEPEKIEP 

       130        140        150        160        170        180 
GEGASRSRRD MLKDGGQQGL GTGARDPGKA TRFRMEELRL TSTTFALTGD SAHNQAMVHW 

       190        200        210        220        230        240 
SGHNSSVILI LTKLYDYNLG SITESSLWRS TDYGTTYEKL NDKVGLKTIL SYLYVCPTNK 

       250        260        270        280        290        300 
CKIMLLTDPE IESSLLISSD EGATYQKYRL NFYLQSLLFH PKQEDWILAY SQDQKLYSSA 

       310        320        330        340        350        360 
EFGRRWQLIQ ESVVPNRFYW SVMGSSKEPD LVHLEARTVD GHSIYLTCRM QNCTEANRNK 

       370        380        390        400        410        420 
PFPGYIDPDS LIVQDDYVFV QLTSGGRPHY YVSYRRSPFA QMKLPKYALP KDMHVISTDE 

       430        440        450        460        470        480 
NQVFAAVQEW NQNDTYNLYI SDTRGVYFTL ALENVRSSRG PEGNVMIDLY EVAGIKGMFL 

       490        500        510        520        530        540 
ANKKIDNQVK TFITYNKGRD WRLLQAPDAD LRGDPVHCLL PYCSLHLHLK VSENPYTSGI 

       550        560        570        580        590        600 
IASRDTAPSI IVASGNIGSE LSDSDISMFV SSDAGNTWRQ IFEEEHSILY LDQGGVLVAM 

       610        620        630        640        650        660 
KHTSLPIRHL WLSFDEGRSW SKYSFTSIPL FVDGVLGEPG EETLIMTVFG HFSHRSEWQL 

       670        680        690        700        710        720 
VKVDYKSIFD RRCAEEDYRP WQLHSQGEAC IMGAKRIYKK RKSERKCMQK YAGAMESEPC 

       730        740        750        760        770        780 
VCTEADFDCD YGYERHSNGQ CLPAFWFNPS SLSKDCSLGQ SYLNSAGYRK VVSNNCTDGV 

       790        800        810        820        830        840 
REQYTAKPQK CPGKAPRGLR IVTADGKLTA EQGHNVTLMV QLEEGDVQRT LIQVDFGDGI 

       850        860        870        880        890        900 
AVSYVNLSSM EDGIKHVYQN VGIFRVTVQV DNSLGSDSAV LYLHVTCPLE HVHLSLPFVT 

       910        920        930        940        950        960 
TKNKEVNATA VLWPSQVGTL TYVWWYGNNT EPLITLEGSI SFKFTSEGMN TITVQVSAGN 

       970        980        990       1000       1010       1020 
AILQDTKTIA VYEEFRSLRL AFSPNLDDYN PDIPEWRRDI SRVIKKSLVE ATGIPSQHIL 

      1030       1040       1050       1060       1070       1080 
VAVLPGLPTA AELFVLPYQD GTRENKRSPE DLEQISEVLI HKLNQNLVHF ELKPGVQVLV 

      1090       1100       1110       1120       1130       1140 
HAAHLTAAPL VDLTPTHSGS AMLMLLSVVF VGLAVFVIYK FKRRVALPSP PSPSAQPGDS 

      1150       1160 
SLRLQRPRPA TPPSSPKRGS AGAQFAI 

References and documentation are available.

Number of amino acids: 1167

Molecular weight: 129631.39

Theoretical pI: 7.35

Amino acid composition: 
Ala (A)  76	  6.5%
Arg (R)  68	  5.8%
Asn (N)  42	  3.6%
Asp (D)  60	  5.1%
Cys (C)  20	  1.7%
Gln (Q)  47	  4.0%
Glu (E)  61	  5.2%
Gly (G)  86	  7.4%
His (H)  31	  2.7%
Ile (I)  52	  4.5%
Leu (L) 117	 10.0%
Lys (K)  53	  4.5%
Met (M)  24	  2.1%
Phe (F)  41	  3.5%
Pro (P)  71	  6.1%
Ser (S) 108	  9.3%
Thr (T)  66	  5.7%
Trp (W)  17	  1.5%
Tyr (Y)  46	  3.9%
Val (V)  81	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 121
Total number of positively charged residues (Arg + Lys): 121

Atomic composition:

Carbon      C	      5772
Hydrogen    H	      9020
Nitrogen    N	      1592
Oxygen      O	      1719
Sulfur      S	        44

Formula: C5772H9020N1592O1719S44
Total number of atoms: 18147

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   163290
Abs 0.1% (=1 g/l)   1.260, assuming all pairs of Cys residues form cystines


Ext. coefficient   162040
Abs 0.1% (=1 g/l)   1.250, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.56
This classifies the protein as unstable.



Aliphatic index: 83.12

Grand average of hydropathicity (GRAVY): -0.288