ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGKVILVTGV SRGIGKSIVD VLFSLDKDTV VYGVARSEAP LKKLKEKYGD RFFYVVGDIT 

        70         80         90        100        110        120 
EDSVLKQLVN AAVKGHGKID SLVANAGVLE PVQNVNEIDV NAWKKLYDIN FFSIVSLVGI 

       130        140        150        160        170        180 
ALPELKKTNG NVVFVSSDAC NMYFSSWGAY GSSKAALNHF AMTLANEERQ VKAIAVAPGI 

       190        200        210        220        230        240 
VDTDMQVNIR ENVGPSSMSA EQLKMFRGLK ENNQLLDSSV PATVYAKLAL HGIPDGVNGQ 

       250 
YLSYNDPALA DFMP 

References and documentation are available.

Number of amino acids: 254

Molecular weight: 27480.55

Theoretical pI: 5.95

Amino acid composition: 
Ala (A)  23	  9.1%
Arg (R)   6	  2.4%
Asn (N)  17	  6.7%
Asp (D)  16	  6.3%
Cys (C)   1	  0.4%
Gln (Q)   7	  2.8%
Glu (E)  11	  4.3%
Gly (G)  20	  7.9%
His (H)   3	  1.2%
Ile (I)  13	  5.1%
Leu (L)  23	  9.1%
Lys (K)  19	  7.5%
Met (M)   7	  2.8%
Phe (F)  10	  3.9%
Pro (P)   9	  3.5%
Ser (S)  20	  7.9%
Thr (T)   7	  2.8%
Trp (W)   2	  0.8%
Tyr (Y)   9	  3.5%
Val (V)  31	 12.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 25

Atomic composition:

Carbon      C	      1234
Hydrogen    H	      1959
Nitrogen    N	       323
Oxygen      O	       369
Sulfur      S	         8

Formula: C1234H1959N323O369S8
Total number of atoms: 3893

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24410
Abs 0.1% (=1 g/l)   0.888, assuming all pairs of Cys residues form cystines


Ext. coefficient    24410
Abs 0.1% (=1 g/l)   0.888, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 24.01
This classifies the protein as stable.



Aliphatic index: 99.72

Grand average of hydropathicity (GRAVY): 0.060