ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGKYMKKSKI TGDISVMEVS KATAPSPGVR TRAAKTLALK RLNSSAADSA LPNDSSCYLQ 

        70         80         90        100        110        120 
LRSRRLEKPS SLIEPKQPPR VHRSGIKESG SRSRVDSVNS VPVAQSSNED ECFDNFVSVQ 

       130        140        150        160        170        180 
VSCGENSLGF ESRHSTREST PCNFVEDMEI MVTPGSSTRS MCRATKEYTR EQDNVIPTTS 

       190        200        210        220 
EMEEFFAYAE QQQQRLFMEK YNFDIVNDIP LSGRYEWVQV KP 

References and documentation are available.

Number of amino acids: 222

Molecular weight: 24924.99

Theoretical pI: 7.60

Amino acid composition: 
Ala (A)  12	  5.4%
Arg (R)  17	  7.7%
Asn (N)  10	  4.5%
Asp (D)  10	  4.5%
Cys (C)   5	  2.3%
Gln (Q)  10	  4.5%
Glu (E)  19	  8.6%
Gly (G)   9	  4.1%
His (H)   2	  0.9%
Ile (I)   8	  3.6%
Leu (L)  11	  5.0%
Lys (K)  13	  5.9%
Met (M)   8	  3.6%
Phe (F)   8	  3.6%
Pro (P)  13	  5.9%
Ser (S)  31	 14.0%
Thr (T)  12	  5.4%
Trp (W)   1	  0.5%
Tyr (Y)   6	  2.7%
Val (V)  17	  7.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1068
Hydrogen    H	      1711
Nitrogen    N	       311
Oxygen      O	       350
Sulfur      S	        13

Formula: C1068H1711N311O350S13
Total number of atoms: 3453

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14690
Abs 0.1% (=1 g/l)   0.589, assuming all pairs of Cys residues form cystines


Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.579, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 61.13
This classifies the protein as unstable.



Aliphatic index: 60.99

Grand average of hydropathicity (GRAVY): -0.678