ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGLCGSKTQP MPSQTTTVAT KARTKPINRD TVKSKQELRH KEKKDKKKKT QLKSTTVPVV 

        70         80         90        100        110        120 
QRKEGSKLTD TSDPSKNKVS PKEAARLAAE KRFQETNEKY NKGELGKKLA QERAKSHKTR 

       130        140 
LMEEAEKKHA ERERENMIYD 

References and documentation are available.

Number of amino acids: 140

Molecular weight: 16037.37

Theoretical pI: 10.03

Amino acid composition: 
Ala (A)  10	  7.1%
Arg (R)  10	  7.1%
Asn (N)   5	  3.6%
Asp (D)   5	  3.6%
Cys (C)   1	  0.7%
Gln (Q)   7	  5.0%
Glu (E)  15	 10.7%
Gly (G)   5	  3.6%
His (H)   3	  2.1%
Ile (I)   2	  1.4%
Leu (L)   8	  5.7%
Lys (K)  27	 19.3%
Met (M)   4	  2.9%
Phe (F)   1	  0.7%
Pro (P)   6	  4.3%
Ser (S)   9	  6.4%
Thr (T)  14	 10.0%
Trp (W)   0	  0.0%
Tyr (Y)   2	  1.4%
Val (V)   6	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 20
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	       683
Hydrogen    H	      1165
Nitrogen    N	       215
Oxygen      O	       218
Sulfur      S	         5

Formula: C683H1165N215O218S5
Total number of atoms: 2286

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     2980
Abs 0.1% (=1 g/l)   0.186, assuming all pairs of Cys residues form cystines


Ext. coefficient     2980
Abs 0.1% (=1 g/l)   0.186, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.78
This classifies the protein as stable.



Aliphatic index: 47.43

Grand average of hydropathicity (GRAVY): -1.483