ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGLDSKEADL EVIRDEKSEA NTVCLHAFSD LTYVSPVVFL YLLKECYKHG SLKATKKFQA 

        70         80         90        100        110        120 
LQYQVHRVLA NKPQPGPATF IINCLTLLPL FGVYGEGFSH LVISALRRFF KTVSEPTSEE 

       130        140        150        160        170        180 
DICLARKLAA QFFLATVGGS LTYDEKVMVH TLRVFDVRLT SIDEALSISE VWQRYGFACG 

       190        200        210        220        230        240 
NAFLEQYISD LIKSKSFMTA VTLLEHFSFR FPGETFLQQM VEDKNFQAAE RWATFMGRPS 

       250        260        270        280        290        300 
LCILVQEYGS RNMLKQAYNI INKNYLQHDF PELYHKCKES ALKVLAEKAC WDVAEIKTKG 

       310        320        330        340        350        360 
DRQLLKYLVY LAVEAGYLEK VDELCDRYSL QGLPKAREAE VAFVEKSFLR LNDLAVEDVV 

       370        380        390        400        410        420 
WVDEVNELRK ATSFLEGCRV VGIDCEWKPN YIKGSKQNKV SIMQIGSDTK IFILDLIKLY 

       430        440        450        460        470        480 
NDASEILDNC LSHILQSKST LKLVSLTEDY PDHKLSSGYN FQCDIKQLAL SYGDLKCFER 

       490        500        510        520        530        540 
YDMLLDIQNV FNEPFGGLAG LTKKILGVSL NKTRRNSDWE QRPLSQNQLE YAALDAAVLI 

       550        560        570        580 
HIFRHVRDHP PHDSSSETTQ WKSHIVSTSY KSPYLSSDNS RR 

References and documentation are available.

Number of amino acids: 582

Molecular weight: 66467.23

Theoretical pI: 6.34

Amino acid composition: 
Ala (A)  38	  6.5%
Arg (R)  26	  4.5%
Asn (N)  22	  3.8%
Asp (D)  34	  5.8%
Cys (C)  14	  2.4%
Gln (Q)  25	  4.3%
Glu (E)  40	  6.9%
Gly (G)  26	  4.5%
His (H)  15	  2.6%
Ile (I)  28	  4.8%
Leu (L)  72	 12.4%
Lys (K)  43	  7.4%
Met (M)   8	  1.4%
Phe (F)  30	  5.2%
Pro (P)  17	  2.9%
Ser (S)  47	  8.1%
Thr (T)  25	  4.3%
Trp (W)   7	  1.2%
Tyr (Y)  25	  4.3%
Val (V)  40	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 74
Total number of positively charged residues (Arg + Lys): 69

Atomic composition:

Carbon      C	      2999
Hydrogen    H	      4681
Nitrogen    N	       787
Oxygen      O	       875
Sulfur      S	        22

Formula: C2999H4681N787O875S22
Total number of atoms: 9364

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    76625
Abs 0.1% (=1 g/l)   1.153, assuming all pairs of Cys residues form cystines


Ext. coefficient    75750
Abs 0.1% (=1 g/l)   1.140, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.84
This classifies the protein as unstable.



Aliphatic index: 93.47

Grand average of hydropathicity (GRAVY): -0.202