ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGLDVEFGAD PASGAAFFQS DGRKHDDLKQ MLDSNKDGLK LEAMKRIIGM IARGRDASDL 

        70         80         90        100        110        120 
FPAVVKNVVS KNIEVKKLVY VYLVRYAEEQ QDLALLSIST FQRALKDPNQ LIRASALRVL 

       130        140        150        160        170        180 
SSIRVSMIVP IVMLAIRDSA ADLSPYVRKT AAHAIPKLYS LDADQKDELV MVIEKLLSDR 

       190        200        210        220        230        240 
TTLVVGSAVM AFDEVCPERV DLIHKNYRKL CNFLLDVDEW GQVIIINMLT RYARTQFVDP 

       250        260        270        280        290        300 
NADDEDLVND GLGETPVSER FYDESSHSSS HSDDGSSDDE KKNKSSTNNK NNGGGNGSRT 

       310        320        330        340        350        360 
PSSPSNSYHI DVDHRLLLRQ TKPLLQSRNA SVVMAVAQLY HHVAPKNEVQ LIAKALIRLL 

       370        380        390        400        410        420 
RSHKEVQSVV LNCIASMSTK RKAIFEPHLK SFFVRTSDPT HIKLLKLDIL TNLASAGSIS 

       430        440        450        460        470        480 
LILREFQTYI SSSDRSFVAA TIQAIGRCAS SIKEVTETCL SGLVHLLSNH DEHVVAESVV 

       490        500        510        520        530        540 
VIKRLLQTKA AEHFEIITQM AKLIDYINVP AARAAIIWLI GEYNEKVPLI APDVLRKMAK 

       550        560        570        580        590        600 
SFVDEQDVVK LQVLNLGVKL YLTNPEQTSL LCQYVFTLAR YDPNYDVRDR ARFLRQIIFP 

       610        620        630        640        650        660 
ASGTSSVLSQ HARQVFLASK PAPVPESKYR DGNNFQLGSL SHYLNMPAAG YKELPAFPVI 

       670        680        690        700        710        720 
PPDSSVRNIA GFMQDKLPGE DSPSGHSKDK SGREGGKEKG AGGEKGFLSE SDDKSSAYSE 

       730        740        750        760        770        780 
SGSSSGSGTS DSESDSDGSG SSDEEEEEVQ KQQPTKVSSV KERLIDAGSN ELAATSSPVK 

       790        800        810        820        830        840 
ATINNNNNAR GSSGTSDSED SSAYSGSSSD DSASGSDNEV KKNETKTDTG TKPEKLKEKH 

       850        860        870        880        890        900 
EKQPEQTAVK SNLDLLLDLD DIPPIGPVMT PSLGGFLTPG KVMRISKLAS LYNNSSIGTP 

       910        920        930        940        950        960 
MMAGQAAPLQ PQHARNRVEL VGPSHIEFKH KELLNKVSGH GLHLAYRFTR SPHLYSSSMC 

       970        980        990       1000       1010       1020 
SIELQFQNRG EKEITAIRLG QTTLPAGMQL NEFAPITVLQ PQQTASGVLG VDFNDSTHAV 

      1030       1040       1050       1060       1070       1080 
DLELLSSAGS SRLQLKPPVG ELVRSVQIGE SCHREERAKL RGMNEHQCEL RGLRQDLIDV 

      1090       1100       1110       1120       1130       1140 
VALRQKVFES INVAHTHSSS SGQLHCFAGQ TLSSKSLVLL TLDWKTEDAL TLLVNCEKMV 

      1150 
IGSMVLNELR NALQLSFAM 

References and documentation are available.

Number of amino acids: 1159

Molecular weight: 126959.84

Theoretical pI: 6.28

Amino acid composition: 
Ala (A)  87	  7.5%
Arg (R)  58	  5.0%
Asn (N)  51	  4.4%
Asp (D)  73	  6.3%
Cys (C)  11	  0.9%
Gln (Q)  50	  4.3%
Glu (E)  70	  6.0%
Gly (G)  68	  5.9%
His (H)  32	  2.8%
Ile (I)  57	  4.9%
Leu (L) 125	 10.8%
Lys (K)  73	  6.3%
Met (M)  25	  2.2%
Phe (F)  33	  2.8%
Pro (P)  50	  4.3%
Ser (S) 134	 11.6%
Thr (T)  49	  4.2%
Trp (W)   3	  0.3%
Tyr (Y)  25	  2.2%
Val (V)  85	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 143
Total number of positively charged residues (Arg + Lys): 131

Atomic composition:

Carbon      C	      5549
Hydrogen    H	      8960
Nitrogen    N	      1574
Oxygen      O	      1755
Sulfur      S	        36

Formula: C5549H8960N1574O1755S36
Total number of atoms: 17874

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    54375
Abs 0.1% (=1 g/l)   0.428, assuming all pairs of Cys residues form cystines


Ext. coefficient    53750
Abs 0.1% (=1 g/l)   0.423, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.92
This classifies the protein as unstable.



Aliphatic index: 90.02

Grand average of hydropathicity (GRAVY): -0.322